Human Progranulin ELISA Kit PicoKine®

Granulin, also known as Proepithelin (PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 507 529 566 377 495 28.05 5.7% Sample 2 1173 1005 874 1096 1037 69.89 6.7% Sample 3 9591 8789 9119 5790 8322 566.75 6.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 507 529 566 377 495 28.05 5.7% Sample 1 507 529 566 377 495 28.05 5.7% Sample 2 1173 1005 874 1096 1037 69.89 6.7% Sample 2 1173 1005 874 1096 1037 69.89 6.7% Sample 3 9591 8789 9119 5790 8322 566.75 6.8% Sample 3 9591 8789 9119 5790 8322 566.75 6.8%

Granulins; Proepithelin; PEPI; Acrogranin; Glycoprotein of 88 Kda; GP88; Glycoprotein 88; Progranulin; Paragranulin; Granulin-1; Granulin G; Granulin-2; Granulin F; Granulin-3; Granulin B; Granulin-4; Granulin A; Granulin-5; Granulin C; Granulin-6; Granulin D; Granulin-7; Granulin E; GRN

In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 507 1173 9591 518 1169 9230 Standard deviation 36.35 71.9 321.3 16.37 88.96 699.63 CV (%) 7.2% 6.1% 3.4% 3.2% 7.6% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 507 1173 9591 518 1169 9230 Standard deviation 36.35 71.9 321.3 16.37 88.96 699.63 CV (%) 7.2% 6.1% 3.4% 3.2% 7.6% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 507 1173 9591 518 1169 9230 Mean (pg/ml) 507 1173 9591 518 1169 9230 Standard deviation 36.35 71.9 321.3 16.37 88.96 699.63 Standard deviation 36.35 71.9 321.3 16.37 88.96 699.63 CV (%) 7.2% 6.1% 3.4% 3.2% 7.6% 7.6% CV (%) 7.2% 6.1% 3.4% 3.2% 7.6% 7.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.039 0.08 0.116 0.221 0.394 0.701 1.15 1.992 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.039 0.08 0.116 0.221 0.394 0.701 1.15 1.992 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.039 0.08 0.116 0.221 0.394 0.701 1.15 1.992 O.D. 0.039 0.08 0.116 0.221 0.394 0.701 1.15 1.992

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.