Human IL-28A ELISA Kit PicoKine®

Interferon lambda-2, also called IFNL2 or IL28A is a class II cytokine receptor ligand related to type I interferons. This gene is mapped to 19q13.2. This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B (IL28B), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster. It up-regulates MHC class I antigen expression. It also displays antitumor activity. The ligand/receptor complex seems to signal through the Jak-STAT pathway. This gene seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR) -induced antiviral defense. It seems play a significant role in the antiviral immune defense in the intestinal epithelium.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 184 181 182 177 8.87 5% Sample 2 1227 1413 1423 1411 1368 81.82 5.9% Sample 3 4870 5325 4875 4906 4994 191.59 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 184 181 182 177 8.87 5% Sample 1 162 184 181 182 177 8.87 5% Sample 2 1227 1413 1423 1411 1368 81.82 5.9% Sample 2 1227 1413 1423 1411 1368 81.82 5.9% Sample 3 4870 5325 4875 4906 4994 191.59 3.8% Sample 3 4870 5325 4875 4906 4994 191.59 3.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 1227 4870 153 1196 5054 Standard deviation 6.8 67.48 282.46 5.46 78.93 343.67 CV (%) 4.2% 5.5% 5.8% 5.4% 6.6% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 1227 4870 153 1196 5054 Standard deviation 6.8 67.48 282.46 5.46 78.93 343.67 CV (%) 4.2% 5.5% 5.8% 5.4% 6.6% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 162 1227 4870 153 1196 5054 Mean (pg/ml) 162 1227 4870 153 1196 5054 Standard deviation 6.8 67.48 282.46 5.46 78.93 343.67 Standard deviation 6.8 67.48 282.46 5.46 78.93 343.67 CV (%) 4.2% 5.5% 5.8% 5.4% 6.6% 6.8% CV (%) 4.2% 5.5% 5.8% 5.4% 6.6% 6.8%

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN- stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type- selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR) -induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.065 0.175 0.252 0.428 0.682 1.08 1.532 2.03 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.065 0.175 0.252 0.428 0.682 1.08 1.532 2.03 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.065 0.175 0.252 0.428 0.682 1.08 1.532 2.03 O.D. 0.065 0.175 0.252 0.428 0.682 1.08 1.532 2.03

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.