Human LYVE-1 ELISA Kit PicoKine®

Extracellular link domain containing 1, also known as LYVE-1 or XLKD1 is a human gene. The International Radiation Hybrid Mapping Consortium mapped the LYVE1 gene to chromosome 11p15.4. This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. This gene may play a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS) . It may act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 86 81 71 80 5.52 6.9% Sample 2 287 265 265 284 275 10.3 3.7% Sample 3 987 829 988 991 948 69.15 7.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 86 81 71 80 5.52 6.9% Sample 1 82 86 81 71 80 5.52 6.9% Sample 2 287 265 265 284 275 10.3 3.7% Sample 2 287 265 265 284 275 10.3 3.7% Sample 3 987 829 988 991 948 69.15 7.2% Sample 3 987 829 988 991 948 69.15 7.2%

Lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE-1; Cell surface retention sequence-binding protein 1; CRSBP-1; Extracellular link domain-containing protein 1; Hyaluronic acid receptor; LYVE1; CRSBP1, HAR, XLKD1; UNQ230/PRO263

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 287 987 78 288 893 Standard deviation 4.59 19.22 52.31 5.85 19.87 63.4 CV (%) 6.7%% 6.7% 5.3% 7.5% 6.9% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 287 987 78 288 893 Standard deviation 4.59 19.22 52.31 5.85 19.87 63.4 CV (%) 6.7%% 6.7% 5.3% 7.5% 6.9% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 82 287 987 78 288 893 Mean (pg/ml) 82 287 987 78 288 893 Standard deviation 4.59 19.22 52.31 5.85 19.87 63.4 Standard deviation 4.59 19.22 52.31 5.85 19.87 63.4 CV (%) 6.7%% 6.7% 5.3% 7.5% 6.9% 7.1% CV (%) 6.7%% 6.7% 5.3% 7.5% 6.9% 7.1%

Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS) . May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Membrane ; Single-pass type I membrane protein . Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand- dependent internalization and recycling at the cell surface.

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.065 0.083 0.103 0.144 0.224 0.406 0.802 1.756 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.065 0.083 0.103 0.144 0.224 0.406 0.802 1.756 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.065 0.083 0.103 0.144 0.224 0.406 0.802 1.756 O.D. 0.065 0.083 0.103 0.144 0.224 0.406 0.802 1.756

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.