Human FSTL1/Tsc 36 ELISA Kit PicoKine®

Human FSTL1/Tsc 36 ELISA Kit PicoKine® (96 Tests) . Quantitate Human FSTL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Follistatin-related protein 1, also called FSTL1 or FRP is a protein that in humans is encoded by the FSTL1 gene. FSTL1 gene is mapped to chromosome 3q13.33 based on an alignment of FSTL1 sequence with the genomic sequence (GRCh37) . This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. This gene may modulate the action of some growth factors on cell proliferation and differentiation. It was suggested that fibroblast-secreted FSTL1 acts on macrophages to increase TNF and IL1B production and IL6 activity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 591 609 629 635 616 17.34 2.8% Sample 2 1511 1639 1624 1771 1636 92.19 5.6% Sample 3 9939 8661 9621 8867 9272 525.36 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 591 609 629 635 616 17.34 2.8% Sample 1 591 609 629 635 616 17.34 2.8% Sample 2 1511 1639 1624 1771 1636 92.19 5.6% Sample 2 1511 1639 1624 1771 1636 92.19 5.6% Sample 3 9939 8661 9621 8867 9272 525.36 5.6% Sample 3 9939 8661 9621 8867 9272 525.36 5.6%

Synonyms

Follistatin-related protein 1; Follistatin-like protein 1; FSTL1; FRP

Gene Name

FSTL1

Gene ID

11167

UniProt

Q12841

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-I308

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Overexpressed in synovial tissues from rheumatoid arthritis. .

Applications

ELISA

Field of Research

Atherosclerosis, Cardiovascular, Cell Biology, Cell Cycle, Cell Differentiation

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 591 1511 9939 616 1368 9532 Standard deviation 26.59 111.81 397.56 38.8 106.7 390.81 CV (%) 4.5% 7.4% 4% 7.8% 7.8% 4.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 591 1511 9939 616 1368 9532 Standard deviation 26.59 111.81 397.56 38.8 106.7 390.81 CV (%) 4.5% 7.4% 4% 7.8% 7.8% 4.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 591 1511 9939 616 1368 9532 Mean (pg/ml) 591 1511 9939 616 1368 9532 Standard deviation 26.59 111.81 397.56 38.8 106.7 390.81 Standard deviation 26.59 111.81 397.56 38.8 106.7 390.81 CV (%) 4.5% 7.4% 4% 7.8% 7.8% 4.1% CV (%) 4.5% 7.4% 4% 7.8% 7.8% 4.1%

Sensitivity

<10 pg/mL

Function

May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

34986 MW

Specificity

Natural and recombinant human FSTL1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Follistatin-related protein 1

Subcellular Location

Secreted .

Sequence Similarities

Contains 2 EF-hand domains.

Protein Name

Follistatin-related protein 1

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.056 0.192 0.247 0.329 0.522 0.868 1.421 1.959 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.056 0.192 0.247 0.329 0.522 0.868 1.421 1.959 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.056 0.192 0.247 0.329 0.522 0.868 1.421 1.959 O.D. 0.056 0.192 0.247 0.329 0.522 0.868 1.421 1.959

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.