Human IGFBP6 ELISA Kit PicoKine®

Insulin-like growth factor-binding protein 6 (IGFBP6) is a protein that in humans is encoded by the IGFBP6 gene. The IGFBP6 gene is mapped to chromosome 12. In all, it has contained 216 amino acid protein with a calculated molecular mass of 22,847, The human keratinocyte cell line HaCat secretes IGFBP6 as an autocrine growth inhibitor. Recombinant IGFBP6 was also shown to inhibit growth of HaCat cells and other keratinocyte cell lines.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 158 152 159 157 3.63 2.3% Sample 2 611 642 578 623 613 23.28 3.7% Sample 3 2123 2028 2170 1875 2049 112.73 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 158 152 159 157 3.63 2.3% Sample 1 162 158 152 159 157 3.63 2.3% Sample 2 611 642 578 623 613 23.28 3.7% Sample 2 611 642 578 623 613 23.28 3.7% Sample 3 2123 2028 2170 1875 2049 112.73 5.5% Sample 3 2123 2028 2170 1875 2049 112.73 5.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 611 2123 160 661 2161 Standard deviation 10.53 40.32 148.61 12.48 46.93 170.71 CV (%) 6.5% 6.6% 7% 7.8% 7.1% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 611 2123 160 661 2161 Standard deviation 10.53 40.32 148.61 12.48 46.93 170.71 CV (%) 6.5% 6.6% 7% 7.8% 7.1% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 162 611 2123 160 661 2161 Mean (pg/ml) 162 611 2123 160 661 2161 Standard deviation 10.53 40.32 148.61 12.48 46.93 170.71 Standard deviation 10.53 40.32 148.61 12.48 46.93 170.71 CV (%) 6.5% 6.6% 7% 7.8% 7.1% 7.9% CV (%) 6.5% 6.6% 7% 7.8% 7.1% 7.9%

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.031 0.105 0.175 0.346 0.669 1.116 1.677 2.073 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.031 0.105 0.175 0.346 0.669 1.116 1.677 2.073 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.031 0.105 0.175 0.346 0.669 1.116 1.677 2.073 O.D. 0.031 0.105 0.175 0.346 0.669 1.116 1.677 2.073

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.