Human PD-1 ELISA Kit PicoKine®

Programmed cell death protein 1, also called PD-1 or PDCD1 is a protein that in humans is encoded by the PDCD1 gene. This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. By fluorescence in situ hybridization, the PDCD1 gene is mapped to 2q37.3. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 25 30 19 23 0.41 1.8% Sample 2 88 94 101 79 91 2.35 2.6% Sample 3 267 294 315 302 295 18.26 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 25 30 19 23 0.41 1.8% Sample 1 17 25 30 19 23 0.41 1.8% Sample 2 88 94 101 79 91 2.35 2.6% Sample 2 88 94 101 79 91 2.35 2.6% Sample 3 267 294 315 302 295 18.26 6.2% Sample 3 267 294 315 302 295 18.26 6.2%

Apoptosis, Associated Proteins, Cell Biology, Developmental Biology, Intracellular, Neurogenesis, Neurology Process, Neuroscience, Organogenesis, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 17 88 267 22 96 314 Standard deviation 0.71 4.49 13.88 1.12 4.99 19.78 CV (%) 4.2% 5.1% 5.2% 5.1% 5.2% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 17 88 267 22 96 314 Standard deviation 0.71 4.49 13.88 1.12 4.99 19.78 CV (%) 4.2% 5.1% 5.2% 5.1% 5.2% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 17 88 267 22 96 314 Mean (pg/ml) 17 88 267 22 96 314 Standard deviation 0.71 4.49 13.88 1.12 4.99 19.78 Standard deviation 0.71 4.49 13.88 1.12 4.99 19.78 CV (%) 4.2% 5.1% 5.2% 5.1% 5.2% 6.3% CV (%) 4.2% 5.1% 5.2% 5.1% 5.2% 6.3%

Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen- specific manner. Possible cell death inducer, in association with other factors. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.161 0.244 0.384 0.652 1.02 1.74 2.611 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.161 0.244 0.384 0.652 1.02 1.74 2.611 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.161 0.244 0.384 0.652 1.02 1.74 2.611 O.D. 0.019 0.161 0.244 0.384 0.652 1.02 1.74 2.611

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.