Human TSLP ELISA Kit PicoKine®

Thymic stromal lymphopoietin, also called TSLP is a protein belonging to the cytokine family. This gene is mapped to 5q22.1. It encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c (+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 89 106 95 91 95 6.57 6.9% Sample 2 239 245 235 240 239 3.56 1.4% Sample 3 883 960 834 974 912 57.16 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 89 106 95 91 95 6.57 6.9% Sample 1 89 106 95 91 95 6.57 6.9% Sample 2 239 245 235 240 239 3.56 1.4% Sample 2 239 245 235 240 239 3.56 1.4% Sample 3 883 960 834 974 912 57.16 6.2% Sample 3 883 960 834 974 912 57.16 6.2%

Isoform 1 is expressed in a number of tissues including heart, liver and prostate. Isoform 2 is the predominant form in keratinocytes of oral mucosa, skin and in salivary glands. It is secreted into saliva. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 239 883 94 243 964 Standard deviation 4.36 17.92 45.21 5.82 19.92 65.55 CV (%) 4.9% 7.5% 5.2% 6.2% 8.2% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 239 883 94 243 964 Standard deviation 4.36 17.92 45.21 5.82 19.92 65.55 CV (%) 4.9% 7.5% 5.2% 6.2% 8.2% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 89 239 883 94 243 964 Mean (pg/ml) 89 239 883 94 243 964 Standard deviation 4.36 17.92 45.21 5.82 19.92 65.55 Standard deviation 4.36 17.92 45.21 5.82 19.92 65.55 CV (%) 4.9% 7.5% 5.2% 6.2% 8.2% 6.8% CV (%) 4.9% 7.5% 5.2% 6.2% 8.2% 6.8%

Isoform 1: Cytokine that induces the release of T-cell- attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c (+) dendritic cells. Can induce allergic inflammation by ly activating mast cells. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.1 0.146 0.222 0.356 0.588 1.016 1.822 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.1 0.146 0.222 0.356 0.588 1.016 1.822 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.1 0.146 0.222 0.356 0.588 1.016 1.822 O.D. 0.055 0.1 0.146 0.222 0.356 0.588 1.016 1.822

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.