Human EPCR ELISA Kit PicoKine®

Endothelial protein C receptor (EPCR) also known as CCCA or PROCR is a protein that in humans is encoded by the PROCR gene. PRPCR gene is mapped to 20q11.2 by radiation hybrid analysis and FISH. The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 678 818 815 809 780 58.97 7.5% Sample 2 1520 1504 1453 1532 1502 30.11 2% Sample 3 9734 8679 10303 9352 9517 590.41 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 678 818 815 809 780 58.97 7.5% Sample 1 678 818 815 809 780 58.97 7.5% Sample 2 1520 1504 1453 1532 1502 30.11 2% Sample 2 1520 1504 1453 1532 1502 30.11 2% Sample 3 9734 8679 10303 9352 9517 590.41 6.2% Sample 3 9734 8679 10303 9352 9517 590.41 6.2%

Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 678 1520 9734 622 1487 10432 Standard deviation 44.07 91.2 486.7 49.13 96.56 678.08 CV (%) 6.5% 6% 5% 7.9% 6.4% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 678 1520 9734 622 1487 10432 Standard deviation 44.07 91.2 486.7 49.13 96.56 678.08 CV (%) 6.5% 6% 5% 7.9% 6.4% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 678 1520 9734 622 1487 10432 Mean (pg/ml) 678 1520 9734 622 1487 10432 Standard deviation 44.07 91.2 486.7 49.13 96.56 678.08 Standard deviation 44.07 91.2 486.7 49.13 96.56 678.08 CV (%) 6.5% 6% 5% 7.9% 6.4% 6.5% CV (%) 6.5% 6% 5% 7.9% 6.4% 6.5%

Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.009 0.136 0.226 0.465 0.762 1.192 1.737 2.053 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.009 0.136 0.226 0.465 0.762 1.192 1.737 2.053 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.009 0.136 0.226 0.465 0.762 1.192 1.737 2.053 O.D. 0.009 0.136 0.226 0.465 0.762 1.192 1.737 2.053

Activate the sample Cell culture supernatants, serum and plasma (heparin, EDTA) : add activating reagent pro rata, i.e. add 25μl of Solution A into 50μl of sample, 10 min later, add 25μl of Solution B. PH 7.0-7.6. It is unnecessary to activate the recombinant EPCR. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration. Activate the sample Cell culture supernatants, serum and plasma (heparin, EDTA) : add activating reagent pro rata, i.e. add 25μl of Solution A into 50μl of sample, 10 min later, add 25μl of Solution B. PH 7.0-7.6. It is unnecessary to activate the recombinant EPCR. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.