Rat EGF ELISA Kit PicoKine®

Rat EGF ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Egf in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Epidermal growth factor (EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays a physiological role in the maintenance of oro-esophageal and gastric tissue integrity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 12 11 11 11 0.43 3.9% Sample 2 90 86 103 93 93 6.28 6.7% Sample 3 232 239 257 243 242 9.12 3.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 12 11 11 11 0.43 3.9% Sample 1 11 12 11 11 11 0.43 3.9% Sample 2 90 86 103 93 93 6.28 6.7% Sample 2 90 86 103 93 93 6.28 6.7% Sample 3 232 239 257 243 242 9.12 3.7% Sample 3 232 239 257 243 242 9.12 3.7%

Synonyms

Pro-epidermal growth factor; EGF; Epidermal growth factor; Egf

Gene Name

EGF

Gene ID

25313

UniProt

P07522

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N974-R1026

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Growth Factors, Growth Factors/Hormones, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

7.8 pg/mL - 500 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 90 232 10 96 211 Standard deviation 0.75 5.31 16.24 0.83 6.91 16.66 CV (%) 6.9% 5.9% 7% 8.3% 7.2% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 90 232 10 96 211 Standard deviation 0.75 5.31 16.24 0.83 6.91 16.66 CV (%) 6.9% 5.9% 7% 8.3% 7.2% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 11 90 232 10 96 211 Mean (pg/ml) 11 90 232 10 96 211 Standard deviation 0.75 5.31 16.24 0.83 6.91 16.66 Standard deviation 0.75 5.31 16.24 0.83 6.91 16.66 CV (%) 6.9% 5.9% 7% 8.3% 7.2% 7.9% CV (%) 6.9% 5.9% 7% 8.3% 7.2% 7.9%

Sensitivity

<1 pg/mL

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

124126 MW

Specificity

Natural and recombinant rat EGF

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Pro-epidermal growth factor

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 9 EGF-like domains.

Protein Name

Pro-epidermal growth factor

Sample Data

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.014 0.059 0.1 0.185 0.349 0.666 1.217 2.03 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.014 0.059 0.1 0.185 0.349 0.666 1.217 2.03 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.014 0.059 0.1 0.185 0.349 0.666 1.217 2.03 O.D. 0.014 0.059 0.1 0.185 0.349 0.666 1.217 2.03

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.