Human MMP-12 ELISA Kit PicoKine®

Matrix metalloproteinase-12 (MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 201 172 181 197 187 11.77 6.2% Sample 2 499 501 511 504 503 4.54 0.9% Sample 3 1029 985 961 957 983 28.63 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 201 172 181 197 187 11.77 6.2% Sample 1 201 172 181 197 187 11.77 6.2% Sample 2 499 501 511 504 503 4.54 0.9% Sample 2 499 501 511 504 503 4.54 0.9% Sample 3 1029 985 961 957 983 28.63 2.9% Sample 3 1029 985 961 957 983 28.63 2.9%

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Tumor Biomarkers

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 201 499 1029 197 507 1084 Standard deviation 12.86 20.45 59.68 13.39 30.42 62.87 CV (%) 6.4% 4.1% 5.8% 6.8% 6% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 201 499 1029 197 507 1084 Standard deviation 12.86 20.45 59.68 13.39 30.42 62.87 CV (%) 6.4% 4.1% 5.8% 6.8% 6% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 201 499 1029 197 507 1084 Mean (pg/ml) 201 499 1029 197 507 1084 Standard deviation 12.86 20.45 59.68 13.39 30.42 62.87 Standard deviation 12.86 20.45 59.68 13.39 30.42 62.87 CV (%) 6.4% 4.1% 5.8% 6.8% 6% 5.8% CV (%) 6.4% 4.1% 5.8% 6.8% 6% 5.8%

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.049 0.158 0.26 0.461 0.789 1.244 1.743 2.163 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.049 0.158 0.26 0.461 0.789 1.244 1.743 2.163 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.049 0.158 0.26 0.461 0.789 1.244 1.743 2.163 O.D. 0.049 0.158 0.26 0.461 0.789 1.244 1.743 2.163

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.