Human Kallikrein 14 ELISA Kit PicoKine®

Kallikrein-14 is a protein that in humans is encoded by the KLK14 gene. Kallikrein-14 belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. Kallikrein-14 gene is identified in the kallikrein gene cluster on chromosome 19q13.4. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. Kallikrein-14 may activate or inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 29 33 31 30 1.92 6.4% Sample 2 211 184 201 205 200 10.03 5% Sample 3 512 489 430 464 473 30.43 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 29 33 31 30 1.92 6.4% Sample 1 28 29 33 31 30 1.92 6.4% Sample 2 211 184 201 205 200 10.03 5% Sample 2 211 184 201 205 200 10.03 5% Sample 3 512 489 430 464 473 30.43 6.4% Sample 3 512 489 430 464 473 30.43 6.4%

Highly expressed in CNS, bone marrow and fetal liver. Also expressed in breast, thyroid, kidney, colon, pancreas, spleen, prostate, uterus, small intestine, placenta and skeletal muscle. Among 40 tissues tested, the highest expression is detected in skin followed by breast and prostate (at protein level) . Expressed in stratum corneum by sweat ducts and sweat glands and detected in sweat (at protein level) . .

Cancer, Cell Biology, Cell Type Markers, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Tags & Cell Markers, Tumor Associated

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 211 512 29 216 500 Standard deviation 2.1 10.12 29.69 2.72 13.39 37.5 CV (%) 7.5% 4.8% 5.8% 9.4% 6.2% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 211 512 29 216 500 Standard deviation 2.1 10.12 29.69 2.72 13.39 37.5 CV (%) 7.5% 4.8% 5.8% 9.4% 6.2% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 211 512 29 216 500 Mean (pg/ml) 28 211 512 29 216 500 Standard deviation 2.1 10.12 29.69 2.72 13.39 37.5 Standard deviation 2.1 10.12 29.69 2.72 13.39 37.5 CV (%) 7.5% 4.8% 5.8% 9.4% 6.2% 7.5% CV (%) 7.5% 4.8% 5.8% 9.4% 6.2% 7.5%

Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.054 0.136 0.216 0.385 0.677 1.185 1.819 2.285 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.054 0.136 0.216 0.385 0.677 1.185 1.819 2.285 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.054 0.136 0.216 0.385 0.677 1.185 1.819 2.285 O.D. 0.054 0.136 0.216 0.385 0.677 1.185 1.819 2.285

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.