Human Endothelin ELISA Kit PicoKine®

The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 13 12 14 12 1.11 9.2% Sample 2 51 49 47 47 48 1.65 3.4% Sample 3 131 117 110 124 120 7.82 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 13 12 14 12 1.11 9.2% Sample 1 11 13 12 14 12 1.11 9.2% Sample 2 51 49 47 47 48 1.65 3.4% Sample 2 51 49 47 47 48 1.65 3.4% Sample 3 131 117 110 124 120 7.82 6.5% Sample 3 131 117 110 124 120 7.82 6.5%

Cancer, Cancer Metabolism, Cardiovascular, Growth Factors/Hormones, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Signal Transduction, Vasculature

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 51 131 11 51 130 Standard deviation 0.62 2.75 8.64 0.62 2.95 10.92 CV (%) 5.7% 5.4% 6.6% 5.7% 5.8% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 51 131 11 51 130 Standard deviation 0.62 2.75 8.64 0.62 2.95 10.92 CV (%) 5.7% 5.4% 6.6% 5.7% 5.8% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 11 51 131 11 51 130 Mean (pg/ml) 11 51 131 11 51 130 Standard deviation 0.62 2.75 8.64 0.62 2.95 10.92 Standard deviation 0.62 2.75 8.64 0.62 2.95 10.92 CV (%) 5.7% 5.4% 6.6% 5.7% 5.8% 8.4% CV (%) 5.7% 5.4% 6.6% 5.7% 5.8% 8.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.022 0.081 0.169 0.336 0.672 1.151 1.597 1.88 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.022 0.081 0.169 0.336 0.672 1.151 1.597 1.88 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.022 0.081 0.169 0.336 0.672 1.151 1.597 1.88 O.D. 0.022 0.081 0.169 0.336 0.672 1.151 1.597 1.88

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.