Human Endothelin ELISA Kit PicoKine®

Human Endothelin ELISA Kit PicoKine® (96 Tests) . Quantitate Human Endothelin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 0.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 13 12 14 12 1.11 9.2% Sample 2 51 49 47 47 48 1.65 3.4% Sample 3 131 117 110 124 120 7.82 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 13 12 14 12 1.11 9.2% Sample 1 11 13 12 14 12 1.11 9.2% Sample 2 51 49 47 47 48 1.65 3.4% Sample 2 51 49 47 47 48 1.65 3.4% Sample 3 131 117 110 124 120 7.82 6.5% Sample 3 131 117 110 124 120 7.82 6.5%

Synonyms

Endothelin-1; Preproendothelin-1; PPET1; Endothelin-1; ET-1; Big endothelin-1; EDN1

Gene Name

EDN1

Gene ID

1906, 1907, 1908

UniProt

P05305, P20800, P14138

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

A peptide reflecting the target protein in human. Sequence: C53-W73

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Monoclonal antibody from mouse

Tissue Specificity

Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. .

Applications

ELISA

Field of Research

Cancer, Cancer Metabolism, Cardiovascular, Growth Factors/Hormones, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Signal Transduction, Vasculature

Standard

1 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

3.9 pg/mL - 250 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 51 131 11 51 130 Standard deviation 0.62 2.75 8.64 0.62 2.95 10.92 CV (%) 5.7% 5.4% 6.6% 5.7% 5.8% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 51 131 11 51 130 Standard deviation 0.62 2.75 8.64 0.62 2.95 10.92 CV (%) 5.7% 5.4% 6.6% 5.7% 5.8% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 11 51 131 11 51 130 Mean (pg/ml) 11 51 131 11 51 130 Standard deviation 0.62 2.75 8.64 0.62 2.95 10.92 Standard deviation 0.62 2.75 8.64 0.62 2.95 10.92 CV (%) 5.7% 5.4% 6.6% 5.7% 5.8% 8.4% CV (%) 5.7% 5.4% 6.6% 5.7% 5.8% 8.4%

Sensitivity

<0.5 pg/mL

Function

Endothelins are endothelium-derived vasoconstrictor peptides.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

24425 MW

Specificity

Natural and recombinant human Endothelin

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Endothelin 1, endothelin 2, endothelin 3

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the endothelin/sarafotoxin family.

Protein Name

Endothelin-1, Endothelin-2, Endothelin-3

Sample Data

Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.022 0.081 0.169 0.336 0.672 1.151 1.597 1.88 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.022 0.081 0.169 0.336 0.672 1.151 1.597 1.88 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.022 0.081 0.169 0.336 0.672 1.151 1.597 1.88 O.D. 0.022 0.081 0.169 0.336 0.672 1.151 1.597 1.88

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.