Human MyD88 ELISA Kit PicoKine®

Human MyD88 ELISA Kit PicoKine® (96 Tests) . Quantitate Human MYD88 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Myeloid differentiation primary response gene (88) (MYD88) is a protein that, in humans, is encoded by the MYD88 gene. By interspecific backcross mapping, the mouse MyD88 gene was localized to chromosome 9; the human homolog was mapped to 3p22-p21.3 The human ortholog MYD88 seems to function similarly to mice, however, available evidence suggests that MYD88 is dispensable for human resistance to common viral infections and to all but a few pyogenic bacterial infections, demonstrating a major difference between mouse and human immune responses.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 244 207 232 211 223 15.17 6.8% Sample 2 985 915 1056 940 974 53.57 5.5% Sample 3 5638 5953 5999 5631 5805 171.54 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 244 207 232 211 223 15.17 6.8% Sample 1 244 207 232 211 223 15.17 6.8% Sample 2 985 915 1056 940 974 53.57 5.5% Sample 2 985 915 1056 940 974 53.57 5.5% Sample 3 5638 5953 5999 5631 5805 171.54 2.9% Sample 3 5638 5953 5999 5631 5805 171.54 2.9%

Synonyms

Myeloid differentiation primary response protein MyD88; MYD88

Gene Name

MYD88

Gene ID

4615

UniProt

Q99836

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-P296

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Ubiquitous. .

Applications

ELISA

Field of Research

Adapters, Atherosclerosis, Cardiovascular, Cytoplasmic, Immunology, Innate Immunity, Signal Transduction, TLR Signaling, Vascular Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 244 985 5638 235 1056 5584 Standard deviation 19.27 61.07 332.64 21.15 76.03 429.96 CV (%) 7.9% 6.2% 5.9% 9% 7.2% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 244 985 5638 235 1056 5584 Standard deviation 19.27 61.07 332.64 21.15 76.03 429.96 CV (%) 7.9% 6.2% 5.9% 9% 7.2% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 244 985 5638 235 1056 5584 Mean (pg/ml) 244 985 5638 235 1056 5584 Standard deviation 19.27 61.07 332.64 21.15 76.03 429.96 Standard deviation 19.27 61.07 332.64 21.15 76.03 429.96 CV (%) 7.9% 6.2% 5.9% 9% 7.2% 7.7% CV (%) 7.9% 6.2% 5.9% 9% 7.2% 7.7%

Sensitivity

<10 pg/mL

Function

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

33233 MW

Specificity

Natural and recombinant human MyD88

Gene Name Synonym

Myeloid differentiation primary response protein MyD88

Subcellular Location

Cytoplasm .

Sequence Similarities

Contains 1 death domain.

Protein Name

Myeloid differentiation primary response protein MyD88

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.065 0.119 0.21 0.369 0.749 1.355 2.007 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.065 0.119 0.21 0.369 0.749 1.355 2.007 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.065 0.119 0.21 0.369 0.749 1.355 2.007 O.D. 0.025 0.065 0.119 0.21 0.369 0.749 1.355 2.007

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.