Human MIA ELISA Kit PicoKine®

Human MIA ELISA Kit PicoKine® (96 Tests) . Quantitate Human MIA in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Melanoma Inhibiting Activity (MIA), also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is an approximately 11-15 kDa protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain (1-3) . And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Beside, It is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta3 induced differentiation into chondrocytes . MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 28 27 29 28 0.82 2.9% Sample 2 172 166 171 170 169 2.27 1.3% Sample 3 485 453 511 458 476 23.22 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 28 27 29 28 0.82 2.9% Sample 1 29 28 27 29 28 0.82 2.9% Sample 2 172 166 171 170 169 2.27 1.3% Sample 2 172 166 171 170 169 2.27 1.3% Sample 3 485 453 511 458 476 23.22 4.8% Sample 3 485 453 511 458 476 23.22 4.8%

Synonyms

Melanoma-derived growth regulatory protein; Melanoma inhibitory activity protein; MIA

Gene Name

MIA

Gene ID

8190

UniProt

Q16674

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G25-131Q

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

All malignant melanoma cell lines tested and infrequently in glioma cell lines.

Applications

ELISA

Field of Research

Cancer, Cytokines, Growth Factors/Hormones, Immunology, Innate Immunity, Secreted Molecules, Signal Transduction, Tumor Immunology

Standard

1 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 172 485 26 165 509 Standard deviation 1.91 13.41 32.98 1.97 15.84 36.92 CV (%) 6.6% 7.8% 6.8% 7.6% 9.6% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 172 485 26 165 509 Standard deviation 1.91 13.41 32.98 1.97 15.84 36.92 CV (%) 6.6% 7.8% 6.8% 7.6% 9.6% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 29 172 485 26 165 509 Mean (pg/ml) 29 172 485 26 165 509 Standard deviation 1.91 13.41 32.98 1.97 15.84 36.92 Standard deviation 1.91 13.41 32.98 1.97 15.84 36.92 CV (%) 6.6% 7.8% 6.8% 7.6% 9.6% 6.9% CV (%) 6.6% 7.8% 6.8% 7.6% 9.6% 6.9%

Sensitivity

<10 pg/mL

Function

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

14509 MW

Specificity

Natural and recombinant human MIA

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Melanoma-derived growth regulatory protein

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the MIA/OTOR family.

Protein Name

Melanoma-derived growth regulatory protein

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.019 0.092 0.162 0.314 0.58 1.017 1.664 2.232 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.019 0.092 0.162 0.314 0.58 1.017 1.664 2.232 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.019 0.092 0.162 0.314 0.58 1.017 1.664 2.232 O.D. 0.019 0.092 0.162 0.314 0.58 1.017 1.664 2.232

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.

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