Human ICAM-3 / CD50 ELISA Kit PicoKine®

Human ICAM-3 / CD50 ELISA Kit PicoKine® (96 Tests) . Quantitate Human ICAM3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50), is a human gene. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. ICAM3 is closely related to ICAM1, consists of 5 immunoglobulin domains, and binds LFA1 through its 2 N-terminal domains. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM3 has been shown to interact with EZR and Moesin.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 378 319 323 361 345 25.02 7.2% Sample 2 1191 1222 1181 1135 1182 31.18 2.6% Sample 3 4088 4762 4153 4037 4260 292.73 6.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 378 319 323 361 345 25.02 7.2% Sample 1 378 319 323 361 345 25.02 7.2% Sample 2 1191 1222 1181 1135 1182 31.18 2.6% Sample 2 1191 1222 1181 1135 1182 31.18 2.6% Sample 3 4088 4762 4153 4037 4260 292.73 6.8% Sample 3 4088 4762 4153 4037 4260 292.73 6.8%

Synonyms

Intercellular adhesion molecule 3; ICAM-3; CDw50; ICAM-R; CD50; ICAM3

Gene Name

ICAM3

Gene ID

3385

UniProt

P32942

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q30-H485; homodimer

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Adapters, Cell Type Markers, Immunology, Protein Phosphorylation, Signal Transduction, Transmembrane

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 378 1191 4088 362 1109 4040 Standard deviation 21.92 58.35 290.24 25.7 57.66 335.32 CV (%) 5.8% 4.9% 7.1% 7.1% 5.2% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 378 1191 4088 362 1109 4040 Standard deviation 21.92 58.35 290.24 25.7 57.66 335.32 CV (%) 5.8% 4.9% 7.1% 7.1% 5.2% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 378 1191 4088 362 1109 4040 Mean (pg/ml) 378 1191 4088 362 1109 4040 Standard deviation 21.92 58.35 290.24 25.7 57.66 335.32 Standard deviation 21.92 58.35 290.24 25.7 57.66 335.32 CV (%) 5.8% 4.9% 7.1% 7.1% 5.2% 8.3% CV (%) 5.8% 4.9% 7.1% 7.1% 5.2% 8.3%

Sensitivity

<10 pg/mL

Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) . ICAM3 is also a ligand for integrin alpha-D/beta-2.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

59541 MW

Specificity

Natural and recombinant human ICAM-3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Intercellular adhesion molecule 3

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the immunoglobulin superfamily. ICAM family.

Protein Name

Intercellular adhesion molecule 3

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.137 0.208 0.355 0.61 1.036 1.567 1.957 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.137 0.208 0.355 0.61 1.036 1.567 1.957 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.137 0.208 0.355 0.61 1.036 1.567 1.957 O.D. 0.042 0.137 0.208 0.355 0.61 1.036 1.567 1.957

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.