Rat sL-Selectin ELISA Kit PicoKine®

L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1 L-selectin acts as a homing receptor for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1474 1304 1342 1320 1360 67.18 4.9% Sample 2 3614 3452 3351 3314 3432 116.19 3.3% Sample 3 24600 22997 23584 22075 23314 916.82 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1474 1304 1342 1320 1360 67.18 4.9% Sample 1 1474 1304 1342 1320 1360 67.18 4.9% Sample 2 3614 3452 3351 3314 3432 116.19 3.3% Sample 2 3614 3452 3351 3314 3432 116.19 3.3% Sample 3 24600 22997 23584 22075 23314 916.82 3.9% Sample 3 24600 22997 23584 22075 23314 916.82 3.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1474 3614 24600 1588 3716 23760 Standard deviation 89.91 166.24 1279.2 100.04 178.36 1663.2 CV (%) 6.1% 4.6% 5.2% 4.8%% 4.8% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1474 3614 24600 1588 3716 23760 Standard deviation 89.91 166.24 1279.2 100.04 178.36 1663.2 CV (%) 6.1% 4.6% 5.2% 4.8%% 4.8% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1474 3614 24600 1588 3716 23760 Mean (pg/ml) 1474 3614 24600 1588 3716 23760 Standard deviation 89.91 166.24 1279.2 100.04 178.36 1663.2 Standard deviation 89.91 166.24 1279.2 100.04 178.36 1663.2 CV (%) 6.1% 4.6% 5.2% 4.8%% 4.8% 7% CV (%) 6.1% 4.6% 5.2% 4.8%% 4.8% 7%

Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.014 0.059 0.1 0.185 0.349 0.666 1.217 2.03 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.014 0.059 0.1 0.185 0.349 0.666 1.217 2.03 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.014 0.059 0.1 0.185 0.349 0.666 1.217 2.03 O.D. 0.014 0.059 0.1 0.185 0.349 0.666 1.217 2.03

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.