Rat sE-Selectin ELISA Kit PicoKine®

E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils.1 An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions.2 E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM) .3 A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1.4

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 345 375 350 321 347 19.17 5.5% Sample 2 1737 1912 2076 1952 1919 121.35 6.3% Sample 3 4428 3889 4055 4258 4157 203.63 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 345 375 350 321 347 19.17 5.5% Sample 1 345 375 350 321 347 19.17 5.5% Sample 2 1737 1912 2076 1952 1919 121.35 6.3% Sample 2 1737 1912 2076 1952 1919 121.35 6.3% Sample 3 4428 3889 4055 4258 4157 203.63 4.8% Sample 3 4428 3889 4055 4258 4157 203.63 4.8%

E-selectin; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD62E; Sele; Elam-1

Cancer, Cofactors, Vitamins/Minerals, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Oncoproteins/Suppressors, Pathways and Processes, Signal Transduction, Tumor Suppressors, Vitamins/Minerals

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 345 1737 4428 315 1621 4077 Standard deviation 17.68 132.01 292.24 14.8 149.13 309.85 CV (%) 4.4% 7.6% 6.6% 4.7% 9.2% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 345 1737 4428 315 1621 4077 Standard deviation 17.68 132.01 292.24 14.8 149.13 309.85 CV (%) 4.4% 7.6% 6.6% 4.7% 9.2% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 345 1737 4428 315 1621 4077 Mean (pg/ml) 345 1737 4428 315 1621 4077 Standard deviation 17.68 132.01 292.24 14.8 149.13 309.85 Standard deviation 17.68 132.01 292.24 14.8 149.13 309.85 CV (%) 4.4% 7.6% 6.6% 4.7% 9.2% 7.6% CV (%) 4.4% 7.6% 6.6% 4.7% 9.2% 7.6%

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.086 0.174 0.331 0.637 1.052 1.614 2.2 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.086 0.174 0.331 0.637 1.052 1.614 2.2 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.086 0.174 0.331 0.637 1.052 1.614 2.2 O.D. 0.008 0.086 0.174 0.331 0.637 1.052 1.614 2.2

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.