Human ADAM12 / MCMP ELISA Kit PicoKine®

ADAM12 (Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 285 300 321 287 298 14.34 4.8% Sample 2 1490 1376 1529 1374 1442 68.65 4.7% Sample 3 4755 4542 4639 5146 4770 229.53 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 285 300 321 287 298 14.34 4.8% Sample 1 285 300 321 287 298 14.34 4.8% Sample 2 1490 1376 1529 1374 1442 68.65 4.7% Sample 2 1490 1376 1529 1374 1442 68.65 4.7% Sample 3 4755 4542 4639 5146 4770 229.53 4.8% Sample 3 4755 4542 4639 5146 4770 229.53 4.8%

Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.

Cancer, Cell Adhesion, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Integrins, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 285 1490 4755 259 1498 4763 Standard deviation 16.81 75.4%9 256.77 19.68 98.86 338.17 CV (%) 5.4%% 7.1%% 5.4% 7.6% 6.6% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 285 1490 4755 259 1498 4763 Standard deviation 16.81 75.4%9 256.77 19.68 98.86 338.17 CV (%) 5.4%% 7.1%% 5.4% 7.6% 6.6% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 285 1490 4755 259 1498 4763 Mean (pg/ml) 285 1490 4755 259 1498 4763 Standard deviation 16.81 75.4%9 256.77 19.68 98.86 338.17 Standard deviation 16.81 75.4%9 256.77 19.68 98.86 338.17 CV (%) 5.4%% 7.1%% 5.4% 7.6% 6.6% 7.1% CV (%) 5.4%% 7.1%% 5.4% 7.6% 6.6% 7.1%

Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.023 0.129 0.235 0.406 0.776 1.301 1.89 2.221 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.023 0.129 0.235 0.406 0.776 1.301 1.89 2.221 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.023 0.129 0.235 0.406 0.776 1.301 1.89 2.221 O.D. 0.023 0.129 0.235 0.406 0.776 1.301 1.89 2.221

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.