Mouse IL-12 (p40) ELISA Kit PicoKine®

Mouse IL-12 (p40) ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Il12b in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-12 (IL12, formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits.1 The larger 40-kDa subunit (p40) is a member of the cytokine receptor family, and the smaller 35-kDa subunit (p35) is related to IL6 and GCSF.2 Both IL-12 p40 (-/-) and p35 (-/-) mice fail to produce IL-12 p70 heterodimer.3 Interleukin (IL) -12 has been cloned on the basis of its ability to activate natural killer (NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon (IFN) -gamma, critical for the initial suppression of bacterial and parasitic infection; for the development of a Th1 response, critical for effective host defense against intracellular pathogens; and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 28 30 33 31 2.12 6.8% Sample 2 233 231 232 259 238 11.71 4.9% Sample 3 425 425 444 461 438 15 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 28 30 33 31 2.12 6.8% Sample 1 33 28 30 33 31 2.12 6.8% Sample 2 233 231 232 259 238 11.71 4.9% Sample 2 233 231 232 259 238 11.71 4.9% Sample 3 425 425 444 461 438 15 3.4% Sample 3 425 425 444 461 438 15 3.4%

Synonyms

Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40; Il12b

Gene Name

IL12B

Gene ID

16160

UniProt

P43432

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M23-S335

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Macrophage/Inflammation, Interleukins

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 233 425 35 253 463 Standard deviation 1.84 13.98 22.52 1.96 17.45 29.63 CV (%) 6% 6% 5.3% 5.6% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 233 425 35 253 463 Standard deviation 1.84 13.98 22.52 1.96 17.45 29.63 CV (%) 6% 6% 5.3% 5.6% 6.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 233 425 35 253 463 Mean (pg/ml) 33 233 425 35 253 463 Standard deviation 1.84 13.98 22.52 1.96 17.45 29.63 Standard deviation 1.84 13.98 22.52 1.96 17.45 29.63 CV (%) 6% 6% 5.3% 5.6% 6.9% 6.4% CV (%) 6% 6% 5.3% 5.6% 6.9% 6.4%

Sensitivity

<10 pg/mL

Function

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

38235 MW

Specificity

Natural and recombinant mouse IL-12 (p40)

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Interleukin-12 subunit beta

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the type I cytokine receptor family. Type 3 subfamily.

Protein Name

Interleukin-12 subunit beta

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.093 0.158 0.271 0.504 0.878 1.344 1.938 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.093 0.158 0.271 0.504 0.878 1.344 1.938 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.093 0.158 0.271 0.504 0.878 1.344 1.938 O.D. 0.023 0.093 0.158 0.271 0.504 0.878 1.344 1.938

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.