Human IL-33 ELISA Kit PicoKine®

Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 superfamily. The IL33 gene maps to chromosome 9p24.1 by using of genomic database analysis. Recombinant mature human IL33 bound to ST2. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell (CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 31 28 32 30 1.47 4.9% Sample 2 260 230 231 235 239 12.26 5.1% Sample 3 484 481 517 495 494 14.13 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 31 28 32 30 1.47 4.9% Sample 1 30 31 28 32 30 1.47 4.9% Sample 2 260 230 231 235 239 12.26 5.1% Sample 2 260 230 231 235 239 12.26 5.1% Sample 3 484 481 517 495 494 14.13 2.8% Sample 3 484 481 517 495 494 14.13 2.8%

Interleukin-33; IL-33; Interleukin-1 family member 11; IL-1F11; Nuclear factor from high endothelial venules; NF-HEV; Interleukin-33 (95-270) ; Interleukin-33 (99-270) ; Interleukin-33 (109-270) ; IL33; C9orf26, IL1F11, NFHEV

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 260 484 28 282 511 Standard deviation 1.74 17.98 37.75 1.76 22.27 47.89 CV (%) 5.8% 7.9% 7.8% 7.9% 7.9% 9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 260 484 28 282 511 Standard deviation 1.74 17.98 37.75 1.76 22.27 47.89 CV (%) 5.8% 7.9% 7.8% 7.9% 7.9% 9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 260 484 28 282 511 Mean (pg/ml) 30 260 484 28 282 511 Standard deviation 1.74 17.98 37.75 1.76 22.27 47.89 Standard deviation 1.74 17.98 37.75 1.76 22.27 47.89 CV (%) 5.8% 7.9% 7.8% 7.9% 7.9% 9% CV (%) 5.8% 7.9% 7.8% 7.9% 7.9% 9%

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016) . Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an alarmin, that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes. Translocation from the nucleus occurs upon biomechanical strain, depends on an intact microtubule network, and is ATP-dependent.

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.078 0.139 0.235 0.409 0.778 1.261 1.834 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.078 0.139 0.235 0.409 0.778 1.261 1.834 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.078 0.139 0.235 0.409 0.778 1.261 1.834 O.D. 0.023 0.078 0.139 0.235 0.409 0.778 1.261 1.834

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.