Human CD244/2B4 ELISA Kit PicoKine®

Human CD244/2B4 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CD244 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead, the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK- and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 27 30 28 28 1.08 3.8% Sample 2 81 79 81 73 78 3.27 4.1% Sample 3 453 479 435 456 455 15.64 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 27 30 28 28 1.08 3.8% Sample 1 28 27 30 28 28 1.08 3.8% Sample 2 81 79 81 73 78 3.27 4.1% Sample 2 81 79 81 73 78 3.27 4.1% Sample 3 453 479 435 456 455 15.64 3.4% Sample 3 453 479 435 456 455 15.64 3.4%

Synonyms

Natural killer cell receptor 2B4; NK cell activation-inducing ligand; NAIL; NK cell type I receptor protein 2B4; NKR2B4; h2B4; SLAM family member 4; SLAMF4; Signaling lymphocytic activation molecule 4; CD244; CD244;2B4

Gene Name

CD244

Gene ID

51744

UniProt

Q9BZW8

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: C22-R221

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils. .

Applications

ELISA

Field of Research

Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, NK Cells, Stem Cells, T Lymphocytic Lineage

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 81 453 30 112 469 Standard deviation 1.34 4.62 20.84 1.53 6.61 30.02 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 81 453 30 112 469 Standard deviation 1.34 4.62 20.84 1.53 6.61 30.02 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 81 453 30 112 469 Mean (pg/ml) 28 81 453 30 112 469 Standard deviation 1.34 4.62 20.84 1.53 6.61 30.02 Standard deviation 1.34 4.62 20.84 1.53 6.61 30.02 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4%

Sensitivity

<10 pg/mL

Function

Modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

41616 MW

Specificity

Natural and recombinant human CD244

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Natural killer cell receptor 2B4

Subcellular Location

Membrane ; Single-pass type I membrane protein .

Protein Name

Natural killer cell receptor 2B4

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.087 0.134 0.235 0.409 0.722 1.329 2.21 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.087 0.134 0.235 0.409 0.722 1.329 2.21 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.087 0.134 0.235 0.409 0.722 1.329 2.21 O.D. 0.032 0.087 0.134 0.235 0.409 0.722 1.329 2.21

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.