Mouse Clusterin / Apolipoprotein J ELISA Kit PicoKine®

Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI) . Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 519 559 591 524 548 29.09 5.3% Sample 2 2684 2395 2417 2667 2540 135.1 5.3% Sample 3 8267 8908 7881 9107 8540 491.35 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 519 559 591 524 548 29.09 5.3% Sample 1 519 559 591 524 548 29.09 5.3% Sample 2 2684 2395 2417 2667 2540 135.1 5.3% Sample 2 2684 2395 2417 2667 2540 135.1 5.3% Sample 3 8267 8908 7881 9107 8540 491.35 5.7% Sample 3 8267 8908 7881 9107 8540 491.35 5.7%

Cancer, Cardiovascular, Chaperones, Complement, Immunology, Innate Immunity, Lipid Metabolism, Lipids/Lipoproteins, Metabolism, Protein Trafficking, Signal Transduction, Tumor Biomarkers

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 519 2684 8267 522 2616 7616 Standard deviation 30.1 112.72 537.35 36.54 122.95 632.12 CV (%) 5.8% 4.2% 6.5% 7% 4.7% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 519 2684 8267 522 2616 7616 Standard deviation 30.1 112.72 537.35 36.54 122.95 632.12 CV (%) 5.8% 4.2% 6.5% 7% 4.7% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 519 2684 8267 522 2616 7616 Mean (pg/ml) 519 2684 8267 522 2616 7616 Standard deviation 30.1 112.72 537.35 36.54 122.95 632.12 Standard deviation 30.1 112.72 537.35 36.54 122.95 632.12 CV (%) 5.8% 4.2% 6.5% 7% 4.7% 8.3% CV (%) 5.8% 4.2% 6.5% 7% 4.7% 8.3%

Functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro) . Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Promotes apoptosis when in the nucleus. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted . Nucleus . Cytoplasm . Mitochondrion membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytosol . Microsome . Endoplasmic reticulum . Cytoplasmic vesicle, secretory vesicle, chromaffin granule . Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis (By similarity) . .

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.029 0.052 0.079 0.152 0.278 0.571 1.162 2.08 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.029 0.052 0.079 0.152 0.278 0.571 1.162 2.08 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.029 0.052 0.079 0.152 0.278 0.571 1.162 2.08 O.D. 0.029 0.052 0.079 0.152 0.278 0.571 1.162 2.08

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.