Human SCGB1A1/uteroglobin ELISA Kit PicoKine®

Human SCGB1A1/uteroglobin ELISA Kit PicoKine® (96 Tests) . Quantitate Human SCGB1A1 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

SCGB1A1 (secretoglobin family 1A member 1) also known as Uteroglobin is a protein that in humans is encoded by the SCGB1A1 gene. SCGB1A1 is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is specifically expressed in Clara cells in the lungs. Numerous studies demonstrated that UG is a multifunctional protein with antiinflammatory/ immunomodulatory properties. It inhibits soluble phospholipase A (2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, UG manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 330 331 310 315 321 9.17 2.8% Sample 2 1779 1757 1732 2013 1820 112.51 6.1% Sample 3 5273 5090 5978 5483 5456 331.91 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 330 331 310 315 321 9.17 2.8% Sample 1 330 331 310 315 321 9.17 2.8% Sample 2 1779 1757 1732 2013 1820 112.51 6.1% Sample 2 1779 1757 1732 2013 1820 112.51 6.1% Sample 3 5273 5090 5978 5483 5456 331.91 6% Sample 3 5273 5090 5978 5483 5456 331.91 6%

Synonyms

Uteroglobin; Clara cell phospholipid-binding protein; CCPBP; Clara cells 10 kDa secretory protein; CC10; Secretoglobin family 1A member 1; Urinary protein 1; UP-1; UP1; Urine protein 1; SCGB1A1; CC10, CCSP, UGB

Gene Name

SCGB1A1

Gene ID

7356

UniProt

P11684

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E22-N91

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Clara cells (nonciliated cells of the surface epithelium of the pulmonary airways) .

Applications

ELISA

Field of Research

Immunoglobulins, Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 330 1779 5273 358 1704 5725 Standard deviation 26.07 124.53 406.02 28.99 119.28 538.15 CV (%) 7.9% 7% 7.7% 8.1% 7% 9.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 330 1779 5273 358 1704 5725 Standard deviation 26.07 124.53 406.02 28.99 119.28 538.15 CV (%) 7.9% 7% 7.7% 8.1% 7% 9.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 330 1779 5273 358 1704 5725 Mean (pg/ml) 330 1779 5273 358 1704 5725 Standard deviation 26.07 124.53 406.02 28.99 119.28 538.15 Standard deviation 26.07 124.53 406.02 28.99 119.28 538.15 CV (%) 7.9% 7% 7.7% 8.1% 7% 9.4% CV (%) 7.9% 7% 7.7% 8.1% 7% 9.4%

Sensitivity

<10 pg/mL

Function

Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

9994 MW

Specificity

Natural and recombinant human SCGB1A1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Uteroglobin

Subcellular Location

Secreted.

Protein Name

Uteroglobin

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.041 0.107 0.181 0.321 0.591 1.128 1.429 2.09 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.041 0.107 0.181 0.321 0.591 1.128 1.429 2.09 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.041 0.107 0.181 0.321 0.591 1.128 1.429 2.09 O.D. 0.041 0.107 0.181 0.321 0.591 1.128 1.429 2.09

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.