Human TNFSF12/Tweak ELISA Kit PicoKine®

Tumor necrosis factor ligand superfamily member 12 also known as TNF-related weak inducer of apoptosis (TWEAK) is a protein that in humans is encoded by the TNFSF12 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator ofangiogenesis. The TNFSF12 gene lies 878 bp upstream of the putative transcriptional start site of the TNFSF13 gene on chromosome 17p13.1.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 160 163 177 179 169 8.34 4.9% Sample 2 584 541 582 513 555 29.7 5.3% Sample 3 1794 2011 2021 1798 1906 110.06 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 160 163 177 179 169 8.34 4.9% Sample 1 160 163 177 179 169 8.34 4.9% Sample 2 584 541 582 513 555 29.7 5.3% Sample 2 584 541 582 513 555 29.7 5.3% Sample 3 1794 2011 2021 1798 1906 110.06 5.7% Sample 3 1794 2011 2021 1798 1906 110.06 5.7%

Tumor necrosis factor ligand superfamily member 12; APO3 ligand; TNF-related weak inducer of apoptosis; TWEAK; Tumor necrosis factor ligand superfamily member 12, membrane form; Tumor necrosis factor ligand superfamily member 12, secreted form; TNFSF12; APO3L, DR3LG; UNQ181/PRO207

Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 160 584 1794 172 587 1626 Standard deviation 9.12 28.61 91.49 12.55 36.98 87.8 CV (%) 5.7% 4.9% 5.4%% 7.3% 6.3% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 160 584 1794 172 587 1626 Standard deviation 9.12 28.61 91.49 12.55 36.98 87.8 CV (%) 5.7% 4.9% 5.4%% 7.3% 6.3% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 160 584 1794 172 587 1626 Mean (pg/ml) 160 584 1794 172 587 1626 Standard deviation 9.12 28.61 91.49 12.55 36.98 87.8 Standard deviation 9.12 28.61 91.49 12.55 36.98 87.8 CV (%) 5.7% 4.9% 5.4%% 7.3% 6.3% 5.4% CV (%) 5.7% 4.9% 5.4%% 7.3% 6.3% 5.4%

Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.051 0.126 0.192 0.338 0.57 1.007 1.474 2.061 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.051 0.126 0.192 0.338 0.57 1.007 1.474 2.061 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.051 0.126 0.192 0.338 0.57 1.007 1.474 2.061 O.D. 0.051 0.126 0.192 0.338 0.57 1.007 1.474 2.061

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.