Human Furin ELISA Kit PicoKine®

Furin (Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 417 420 397 384 404 14.77 3.6% Sample 2 2751 2551 2738 2985 2756 153.96 5.5% Sample 3 8293 8993 7686 9246 8554 610.96 7.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 417 420 397 384 404 14.77 3.6% Sample 1 417 420 397 384 404 14.77 3.6% Sample 2 2751 2551 2738 2985 2756 153.96 5.5% Sample 2 2751 2551 2738 2985 2756 153.96 5.5% Sample 3 8293 8993 7686 9246 8554 610.96 7.1% Sample 3 8293 8993 7686 9246 8554 610.96 7.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 417 2751 82701 457 2701 7870 Standard deviation 21.68 143.05 464.4 28.79 148.55 448.59 CV (%) 5.2% 5.2% 5.6% 5.5%% 5.5% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 417 2751 82701 457 2701 7870 Standard deviation 21.68 143.05 464.4 28.79 148.55 448.59 CV (%) 5.2% 5.2% 5.6% 5.5%% 5.5% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 417 2751 82701 457 2701 7870 Mean (pg/ml) 417 2751 82701 457 2701 7870 Standard deviation 21.68 143.05 464.4 28.79 148.55 448.59 Standard deviation 21.68 143.05 464.4 28.79 148.55 448.59 CV (%) 5.2% 5.2% 5.6% 5.5%% 5.5% 5.7% CV (%) 5.2% 5.2% 5.6% 5.5%% 5.5% 5.7%

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER) . A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin.

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.03 0.111 0.197 0.369 0.68 1.252 1.783 2.15 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.03 0.111 0.197 0.369 0.68 1.252 1.783 2.15 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.03 0.111 0.197 0.369 0.68 1.252 1.783 2.15 O.D. 0.03 0.111 0.197 0.369 0.68 1.252 1.783 2.15

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.