Mouse CD25/IL-2sR Alpha ELISA Kit PicoKine®

CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 143 134 137 152 141 6.87 4.8% Sample 2 707 650 699 681 684 21.9 3.2% Sample 3 2735 2916 2595 2589 2708 133.15 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 143 134 137 152 141 6.87 4.8% Sample 1 143 134 137 152 141 6.87 4.8% Sample 2 707 650 699 681 684 21.9 3.2% Sample 2 707 650 699 681 684 21.9 3.2% Sample 3 2735 2916 2595 2589 2708 133.15 4.9% Sample 3 2735 2916 2595 2589 2708 133.15 4.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 143 707 2735 152 647 2781 Standard deviation 7.29 51.61 142.22 9.72 49.81 194.67 CV (%) 7%% 7.3% 5.2% 6.4% 7.7% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 143 707 2735 152 647 2781 Standard deviation 7.29 51.61 142.22 9.72 49.81 194.67 CV (%) 7%% 7.3% 5.2% 6.4% 7.7% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 143 707 2735 152 647 2781 Mean (pg/ml) 143 707 2735 152 647 2781 Standard deviation 7.29 51.61 142.22 9.72 49.81 194.67 Standard deviation 7.29 51.61 142.22 9.72 49.81 194.67 CV (%) 7%% 7.3% 5.2% 6.4% 7.7% 7% CV (%) 7%% 7.3% 5.2% 6.4% 7.7% 7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.095 0.132 0.159 0.232 0.337 0.568 1.079 1.882 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.095 0.132 0.159 0.232 0.337 0.568 1.079 1.882 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.095 0.132 0.159 0.232 0.337 0.568 1.079 1.882 O.D. 0.095 0.132 0.159 0.232 0.337 0.568 1.079 1.882

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.