Mouse CD25/IL-2sR Alpha ELISA Kit PicoKine®

Mouse CD25/IL-2sR Alpha ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Il2ra in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 143 134 137 152 141 6.87 4.8% Sample 2 707 650 699 681 684 21.9 3.2% Sample 3 2735 2916 2595 2589 2708 133.15 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 143 134 137 152 141 6.87 4.8% Sample 1 143 134 137 152 141 6.87 4.8% Sample 2 707 650 699 681 684 21.9 3.2% Sample 2 707 650 699 681 684 21.9 3.2% Sample 3 2735 2916 2595 2589 2708 133.15 4.9% Sample 3 2735 2916 2595 2589 2708 133.15 4.9%

Synonyms

Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; p55; CD25; Il2ra; Il2r

Gene Name

IL2RA

Gene ID

16184

UniProt

P01590

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-K236

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Adaptive Immunity, Cytokines, Immunology, Innate Immunity, Interleukins, T Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 143 707 2735 152 647 2781 Standard deviation 7.29 51.61 142.22 9.72 49.81 194.67 CV (%) 7%% 7.3% 5.2% 6.4% 7.7% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 143 707 2735 152 647 2781 Standard deviation 7.29 51.61 142.22 9.72 49.81 194.67 CV (%) 7%% 7.3% 5.2% 6.4% 7.7% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 143 707 2735 152 647 2781 Mean (pg/ml) 143 707 2735 152 647 2781 Standard deviation 7.29 51.61 142.22 9.72 49.81 194.67 Standard deviation 7.29 51.61 142.22 9.72 49.81 194.67 CV (%) 7%% 7.3% 5.2% 6.4% 7.7% 7% CV (%) 7%% 7.3% 5.2% 6.4% 7.7% 7%

Sensitivity

<5 pg/mL

Function

Receptor for interleukin-2.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

30723 MW

Specificity

Natural and recombinant mouse CD25

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-2 receptor subunit alpha

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

Interleukin-2 receptor subunit alpha

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.095 0.132 0.159 0.232 0.337 0.568 1.079 1.882 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.095 0.132 0.159 0.232 0.337 0.568 1.079 1.882 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.095 0.132 0.159 0.232 0.337 0.568 1.079 1.882 O.D. 0.095 0.132 0.159 0.232 0.337 0.568 1.079 1.882

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.