Human COMP/Thrombospondin 5 ELISA Kit PicoKine®

Cartilage oligomeric matrix protein is a protein that in humans is encoded by the COMP gene. The sequences of rat and bovine COMP indicate that it is a member of the thrombospondin gene family. By Southern blot analysis of a somatic cell hybrid DNA panel and by isotopic in situ hybridization, human COMP gene was mapped to 19p13.1, and the murine COMP gene was mapped to the central region of mouse chromosome 8 by use of an interspecific backcross mapping panel. COMP is a marker of cartilage turnover.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 233 203 234 223 223 12.45 5.5% Sample 2 1997 2142 1949 1828 1979 112.46 5.6% Sample 3 4935 5185 4588 4986 4923 215.04 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 233 203 234 223 223 12.45 5.5% Sample 1 233 203 234 223 223 12.45 5.5% Sample 2 1997 2142 1949 1828 1979 112.46 5.6% Sample 2 1997 2142 1949 1828 1979 112.46 5.6% Sample 3 4935 5185 4588 4986 4923 215.04 4.3% Sample 3 4935 5185 4588 4986 4923 215.04 4.3%

Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. Increased amounts are produced during late stages of osteoarthritis in the area adjacent to the main defect. .

Cell Biology, Cell Cycle, Cell Differentiation, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells, Structures

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 233 1997 4935 255 1893 5413 Standard deviation 9.78 137.79 246.75 12.49 138.18 281.47 CV (%) 4.2% 6.9% 5% 4.9% 7.3% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 233 1997 4935 255 1893 5413 Standard deviation 9.78 137.79 246.75 12.49 138.18 281.47 CV (%) 4.2% 6.9% 5% 4.9% 7.3% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 233 1997 4935 255 1893 5413 Mean (pg/ml) 233 1997 4935 255 1893 5413 Standard deviation 9.78 137.79 246.75 12.49 138.18 281.47 Standard deviation 9.78 137.79 246.75 12.49 138.18 281.47 CV (%) 4.2% 6.9% 5% 4.9% 7.3% 5.2% CV (%) 4.2% 6.9% 5% 4.9% 7.3% 5.2%

May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP) . Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.02 0.066 0.12 0.231 0.462 0.918 1.816 2.572 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.02 0.066 0.12 0.231 0.462 0.918 1.816 2.572 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.02 0.066 0.12 0.231 0.462 0.918 1.816 2.572 O.D. 0.02 0.066 0.12 0.231 0.462 0.918 1.816 2.572

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.