Human TLR3/Toll-like receptor 3 ELISA Kit PicoKine®

Toll-like receptor 3 (TLR3) also known as CD283 (cluster of differentiation 283) is a protein that in humans is encoded by the TLR3 gene. The TLR3 gene was mapped to chromosome 4q35 by fluorescence in situ hybridization. TLR3 specifically recognizes double-stranded RNA (dsRNA) and induces multiple intracellular events responsible for innate antiviral immunity against a number of viral infections.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 290 307 297 293 296 6.41 2.1% Sample 2 1771 1687 1955 1718 1782 103.88 5.8% Sample 3 5327 6029 6362 5287 5751 459.8 7.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 290 307 297 293 296 6.41 2.1% Sample 1 290 307 297 293 296 6.41 2.1% Sample 2 1771 1687 1955 1718 1782 103.88 5.8% Sample 2 1771 1687 1955 1718 1782 103.88 5.8% Sample 3 5327 6029 6362 5287 5751 459.8 7.9% Sample 3 5327 6029 6362 5287 5751 459.8 7.9%

Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. .

Chemokines, Host-Virus Interaction, Immune System Diseases, Immunoglobulins, Immunology, Innate Immunity, Interspecies Interaction, Macrophage/Inflammation, Microbiology, NFKB Pathway, Nuclear Signaling, Receptors, Signal Transduction, Signaling Pathway, TLR Signaling

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 290 1771 5327 301 1733 5141 Standard deviation 14.21 125.74 292.98 18.96 152.5 374.9%9 CV (%) 4.9% 7.1% 5.5% 8.8%% 8.8% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 290 1771 5327 301 1733 5141 Standard deviation 14.21 125.74 292.98 18.96 152.5 374.9%9 CV (%) 4.9% 7.1% 5.5% 8.8%% 8.8% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 290 1771 5327 301 1733 5141 Mean (pg/ml) 290 1771 5327 301 1733 5141 Standard deviation 14.21 125.74 292.98 18.96 152.5 374.9%9 Standard deviation 14.21 125.74 292.98 18.96 152.5 374.9%9 CV (%) 4.9% 7.1% 5.5% 8.8%% 8.8% 7.3% CV (%) 4.9% 7.1% 5.5% 8.8%% 8.8% 7.3%

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.06 0.134 0.206 0.373 0.66 1.049 1.574 2.038 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.06 0.134 0.206 0.373 0.66 1.049 1.574 2.038 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.06 0.134 0.206 0.373 0.66 1.049 1.574 2.038 O.D. 0.06 0.134 0.206 0.373 0.66 1.049 1.574 2.038

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.