Human TLR2/Toll-like receptor 2 ELISA Kit PicoKine®

Toll-like receptor 2 also known as TLR-2 is a protein that in humans is encoded by the TLR2 gene. TLR2 has also been designated as CD282 (cluster of differentiation 282) . TLR-2 plays a role in the immune system. TLR-2 is a membrane protein, a receptor, which is expressed on the surface of certain cells and recognizes foreign substances and passes on appropriate signals to the cells of the immune system. TLR2 expresses as 4- and 4.4-kb mRNAs in heart, brain, and muscle..

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 369 373 338 372 363 14.5 3.9% Sample 2 2018 1834 1789 2014 1913 103.49 5.4% Sample 3 5303 5820 5768 5756 5661 208.51 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 369 373 338 372 363 14.5 3.9% Sample 1 369 373 338 372 363 14.5 3.9% Sample 2 2018 1834 1789 2014 1913 103.49 5.4% Sample 2 2018 1834 1789 2014 1913 103.49 5.4% Sample 3 5303 5820 5768 5756 5661 208.51 3.6% Sample 3 5303 5820 5768 5756 5661 208.51 3.6%

Adapters, Atherosclerosis, Cardiovascular, Defensin, Human Protein, Immunoglobulins, Immunology, Innate Immunity, Macrophage/Inflammation, Microbiology, Protein, Receptors, Signal Transduction, TLR Signaling, Transmembrane, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 369 2018 5303 392 1927 5643 Standard deviation 18.81 133.18 212.12 22.34 150.3 242.64 CV (%) 4.3% 6.6% 4% 5.7% 7.8% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 369 2018 5303 392 1927 5643 Standard deviation 18.81 133.18 212.12 22.34 150.3 242.64 CV (%) 4.3% 6.6% 4% 5.7% 7.8% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 369 2018 5303 392 1927 5643 Mean (pg/ml) 369 2018 5303 392 1927 5643 Standard deviation 18.81 133.18 212.12 22.34 150.3 242.64 Standard deviation 18.81 133.18 212.12 22.34 150.3 242.64 CV (%) 4.3% 6.6% 4% 5.7% 7.8% 4.3% CV (%) 4.3% 6.6% 4% 5.7% 7.8% 4.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.059 0.11 0.232 0.404 0.831 1.479 2.061 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.059 0.11 0.232 0.404 0.831 1.479 2.061 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.059 0.11 0.232 0.404 0.831 1.479 2.061 O.D. 0.004 0.059 0.11 0.232 0.404 0.831 1.479 2.061

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.