Human Angiostatin Kringle 1-3/PLG ELISA Kit PicoKine®

Human Angiostatin Kringle 1-3/PLG ELISA Kit PicoKine® (96 Tests) . Quantitate Human PLG in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity (ED?? =70 nM) relative to kringles 1-4 (ED?? = 135 nM) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 241 249 248 219 239 12.09 5% Sample 2 1987 1892 1804 1733 1854 95.22 5.1% Sample 3 5716 5780 5230 5301 5506 243.6 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 241 249 248 219 239 12.09 5% Sample 1 241 249 248 219 239 12.09 5% Sample 2 1987 1892 1804 1733 1854 95.22 5.1% Sample 2 1987 1892 1804 1733 1854 95.22 5.1% Sample 3 5716 5780 5230 5301 5506 243.6 4.4% Sample 3 5716 5780 5230 5301 5506 243.6 4.4%

Synonyms

Plasminogen;3.4.21.7; Plasmin heavy chain A; Activation peptide; Angiostatin; Plasmin heavy chain A, short form; Plasmin light chain B; PLG

Gene Name

PLG

Gene ID

5340

UniProt

P00747

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V98-S355 (K1-3)

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Present in plasma and many other extracellular fluids. It is synthesized in the liver.

Applications

ELISA

Field of Research

Cardiovascular, Cell Biology, Platelets, Protease Inhibitors, Proteolysis/Ubiquitin, Proteolytic Enzymes

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 241 1987 5716 218 1908 5845 Standard deviation 12.05 79.48 337.24 13.73 91.58 432.53 CV (%) 5% 4% 5.9% 4.8% 4.8% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 241 1987 5716 218 1908 5845 Standard deviation 12.05 79.48 337.24 13.73 91.58 432.53 CV (%) 5% 4% 5.9% 4.8% 4.8% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 241 1987 5716 218 1908 5845 Mean (pg/ml) 241 1987 5716 218 1908 5845 Standard deviation 12.05 79.48 337.24 13.73 91.58 432.53 Standard deviation 12.05 79.48 337.24 13.73 91.58 432.53 CV (%) 5% 4% 5.9% 4.8% 4.8% 7.4% CV (%) 5% 4% 5.9% 4.8% 4.8% 7.4%

Sensitivity

<10 pg/mL

Function

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

90569 MW

Specificity

Natural and recombinant human Angiostatin K1-3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Plasminogen

Subcellular Location

Secreted . Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.

Sequence Similarities

Belongs to the peptidase S1 family. Plasminogen subfamily.

Protein Name

Plasminogen

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.11 0.182 0.328 0.575 0.969 1.498 2.042 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.11 0.182 0.328 0.575 0.969 1.498 2.042 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.11 0.182 0.328 0.575 0.969 1.498 2.042 O.D. 0.044 0.11 0.182 0.328 0.575 0.969 1.498 2.042

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.