Human KLK1/Kallikrein 1 ELISA Kit PicoKine®

Human KLK1/Kallikrein 1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human KLK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 304 305 265 265 284 19.75 6.9% Sample 2 1654 1642 1403 1384 1520 127.49 8.3% Sample 3 3697 4059 4071 4283 4027 210.59 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 304 305 265 265 284 19.75 6.9% Sample 1 304 305 265 265 284 19.75 6.9% Sample 2 1654 1642 1403 1384 1520 127.49 8.3% Sample 2 1654 1642 1403 1384 1520 127.49 8.3% Sample 3 3697 4059 4071 4283 4027 210.59 5.2% Sample 3 3697 4059 4071 4283 4027 210.59 5.2%

Synonyms

Kallikrein-1;3.4.21.35; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein; KLK1

Gene Name

KLK1

Gene ID

3816

UniProt

P06870

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I25-S262

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84. .

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Serum Proteins, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 304 1654 3697 280 1605 3442 Standard deviation 12.46 71.12 284.66 16.24 93.09 271.91 CV (%) 4.1% 4.3% 7.7% 5.8% 5.8% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 304 1654 3697 280 1605 3442 Standard deviation 12.46 71.12 284.66 16.24 93.09 271.91 CV (%) 4.1% 4.3% 7.7% 5.8% 5.8% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 304 1654 3697 280 1605 3442 Mean (pg/ml) 304 1654 3697 280 1605 3442 Standard deviation 12.46 71.12 284.66 16.24 93.09 271.91 Standard deviation 12.46 71.12 284.66 16.24 93.09 271.91 CV (%) 4.1% 4.3% 7.7% 5.8% 5.8% 7.9% CV (%) 4.1% 4.3% 7.7% 5.8% 5.8% 7.9%

Sensitivity

<10 pg/mL

Function

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

28890 MW

Specificity

Natural and recombinant human KLK1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Kallikrein-1

Sequence Similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein Name

Kallikrein-1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.087 0.169 0.306 0.5438 0.938 1.364 1.849 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.087 0.169 0.306 0.5438 0.938 1.364 1.849 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.087 0.169 0.306 0.5438 0.938 1.364 1.849 O.D. 0.017 0.087 0.169 0.306 0.5438 0.938 1.364 1.849

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.