Rat MCP-1 ELISA Kit PicoKine®

Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 41 37 39 42 39 1.92 4.9% Sample 2 257 231 216 228 233 14.94 6.4% Sample 3 473 462 446 461 460 9.6 2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 41 37 39 42 39 1.92 4.9% Sample 1 41 37 39 42 39 1.92 4.9% Sample 2 257 231 216 228 233 14.94 6.4% Sample 2 257 231 216 228 233 14.94 6.4% Sample 3 473 462 446 461 460 9.6 2% Sample 3 473 462 446 461 460 9.6 2%

C-C motif chemokine 2; Immediate-early serum-responsive protein JE; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; MCP-1; Small-inducible cytokine A2; CCL2; Je, Mcp1, Scya2

Angiogenesis, Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Chemokines, Heart Disease, Immunology, Innate Immunity, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 41 257 473 40 251 504 Standard deviation 2.29 21.58 27.43 2.56 21.58 29.23 CV (%) 7.2% 7.2% 5.8% 6.4% 8.6% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 41 257 473 40 251 504 Standard deviation 2.29 21.58 27.43 2.56 21.58 29.23 CV (%) 7.2% 7.2% 5.8% 6.4% 8.6% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 41 257 473 40 251 504 Mean (pg/ml) 41 257 473 40 251 504 Standard deviation 2.29 21.58 27.43 2.56 21.58 29.23 Standard deviation 2.29 21.58 27.43 2.56 21.58 29.23 CV (%) 7.2% 7.2% 5.8% 6.4% 8.6% 5.8% CV (%) 7.2% 7.2% 5.8% 6.4% 8.6% 5.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles. (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.168 0.271 0.454 0.859 1.286 1.79 2.13 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.168 0.271 0.454 0.859 1.286 1.79 2.13 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.168 0.271 0.454 0.859 1.286 1.79 2.13 O.D. 0.007 0.168 0.271 0.454 0.859 1.286 1.79 2.13

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.