Human THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine®

Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to human 15q15 and the cognate gene to mouse chromosome 2 (region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2414 2383 2098 2136 2257 141.81 6.2% Sample 2 13444 12251 11532 12803 12507 703.87 5.6% Sample 3 38908 44108 38626 45595 41809 3088.95 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2414 2383 2098 2136 2257 141.81 6.2% Sample 1 2414 2383 2098 2136 2257 141.81 6.2% Sample 2 13444 12251 11532 12803 12507 703.87 5.6% Sample 2 13444 12251 11532 12803 12507 703.87 5.6% Sample 3 38908 44108 38626 45595 41809 3088.95 7.3% Sample 3 38908 44108 38626 45595 41809 3088.95 7.3%

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cell Adhesion Proteins, Cell Type Marker, Invasion/Microenvironment, Metabolism, Metabolism Processes, Neuroscience, Pathways and Processes, Platelets, Response To Hypoxia, Secreted, Signaling Pathways, Stem Cells, TGF Beta

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2414 13444 38908 2523 13902 38494 Standard deviation 152.08 994.85 2451.2 176.61 1278.98 2771.56 CV (%) 9.2%% 7.4% 9.2%% 7% 9.2% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2414 13444 38908 2523 13902 38494 Standard deviation 152.08 994.85 2451.2 176.61 1278.98 2771.56 CV (%) 9.2%% 7.4% 9.2%% 7% 9.2% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2414 13444 38908 2523 13902 38494 Mean (pg/ml) 2414 13444 38908 2523 13902 38494 Standard deviation 152.08 994.85 2451.2 176.61 1278.98 2771.56 Standard deviation 152.08 994.85 2451.2 176.61 1278.98 2771.56 CV (%) 9.2%% 7.4% 9.2%% 7% 9.2% 7.2% CV (%) 9.2%% 7.4% 9.2%% 7% 9.2% 7.2%

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity) . Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.029 0.098 0.157 0.298 0.574 0.994 1.689 2.367 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.029 0.098 0.157 0.298 0.574 0.994 1.689 2.367 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.029 0.098 0.157 0.298 0.574 0.994 1.689 2.367 O.D. 0.029 0.098 0.157 0.298 0.574 0.994 1.689 2.367

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.