Human P53 ELISA Kit PicoKine®

P53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kilodalton (kDa) protein on SDS-PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full-length p53. It has played a vital role in conserving stability by preventing genome mutation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 275 249 256 233 253 15.07 5.9% Sample 2 1540 1645 1702 1738 1656 74.86 4.5% Sample 3 5055 4442 4533 4238 4567 301.31 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 275 249 256 233 253 15.07 5.9% Sample 1 275 249 256 233 253 15.07 5.9% Sample 2 1540 1645 1702 1738 1656 74.86 4.5% Sample 2 1540 1645 1702 1738 1656 74.86 4.5% Sample 3 5055 4442 4533 4238 4567 301.31 6.5% Sample 3 5055 4442 4533 4238 4567 301.31 6.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 275 1540 5055 302 1396 5437 Standard deviation 16.5 72.38 394.29 20.83 71.19 467.58 CV (%) 6% 4.7% 7.8% 6.9% 8.6% 8.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 275 1540 5055 302 1396 5437 Standard deviation 16.5 72.38 394.29 20.83 71.19 467.58 CV (%) 6% 4.7% 7.8% 6.9% 8.6% 8.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 275 1540 5055 302 1396 5437 Mean (pg/ml) 275 1540 5055 302 1396 5437 Standard deviation 16.5 72.38 394.29 20.83 71.19 467.58 Standard deviation 16.5 72.38 394.29 20.83 71.19 467.58 CV (%) 6% 4.7% 7.8% 6.9% 8.6% 8.6% CV (%) 6% 4.7% 7.8% 6.9% 8.6% 8.6%

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.088 0.127 0.207 0.363 0.674 1.198 2.028 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.088 0.127 0.207 0.363 0.674 1.198 2.028 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.088 0.127 0.207 0.363 0.674 1.198 2.028 O.D. 0.044 0.088 0.127 0.207 0.363 0.674 1.198 2.028

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.