Human Endostatin ELISA Kit PicoKine®

Human Endostatin ELISA Kit PicoKine® (96 Tests) . Quantitate Human COL18A1 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And it is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 238 232 204 230 16.06 6.9% Sample 2 1523 1309 1362 1479 1418 86.25 6% Sample 3 5144 5141 5851 5778 5478 336.99 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 247 238 232 204 230 16.06 6.9% Sample 1 247 238 232 204 230 16.06 6.9% Sample 2 1523 1309 1362 1479 1418 86.25 6% Sample 2 1523 1309 1362 1479 1418 86.25 6% Sample 3 5144 5141 5851 5778 5478 336.99 6.1% Sample 3 5144 5141 5851 5778 5478 336.99 6.1%

Synonyms

Collagen alpha-1 (XVIII) chain; Endostatin; COL18A1

Gene Name

COL18A1

Gene ID

80781

UniProt

P39060

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H1154-S1335

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Present in multiple organs with highest levels in liver, lung and kidney.

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Collagen, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1523 5144 251 1462 5243 Standard deviation 14.07 109.65 216.04 14.3 130.11 319.82 CV (%) 5.7% 7.2% 4.2% 5.7% 8.9% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 247 1523 5144 251 1462 5243 Standard deviation 14.07 109.65 216.04 14.3 130.11 319.82 CV (%) 5.7% 7.2% 4.2% 5.7% 8.9% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 247 1523 5144 251 1462 5243 Mean (pg/ml) 247 1523 5144 251 1462 5243 Standard deviation 14.07 109.65 216.04 14.3 130.11 319.82 Standard deviation 14.07 109.65 216.04 14.3 130.11 319.82 CV (%) 5.7% 7.2% 4.2% 5.7% 8.9% 6.1% CV (%) 5.7% 7.2% 4.2% 5.7% 8.9% 6.1%

Sensitivity

<10 pg/mL

Function

COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

178188 MW

Specificity

Natural and recombinant human Endostatin

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Collagen alpha-1 (XVIII) chain

Subcellular Location

Secreted, extracellular space, extracellular matrix .

Sequence Similarities

Belongs to the multiplexin collagen family.

Protein Name

Collagen alpha-1 (XVIII) chain

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.047 0.095 0.149 0.245 0.432 0.77 1.308 1.98 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.047 0.095 0.149 0.245 0.432 0.77 1.308 1.98 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.047 0.095 0.149 0.245 0.432 0.77 1.308 1.98 O.D. 0.047 0.095 0.149 0.245 0.432 0.77 1.308 1.98

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.