Human KIM1 / TIM-1 ELISA Kit PicoKine®

Human KIM1 / TIM-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human HAVCR1 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rats is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to Rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the Rat Tim1 gene and database analysis, the HAVCR1 gene was mapped to 5q33.2.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 93 93 91 92 0.82 0.8% Sample 2 215 235 212 211 218 9.78 4.4% Sample 3 880 798 777 920 843 58.47 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 93 93 91 92 0.82 0.8% Sample 1 92 93 93 91 92 0.82 0.8% Sample 2 215 235 212 211 218 9.78 4.4% Sample 2 215 235 212 211 218 9.78 4.4% Sample 3 880 798 777 920 843 58.47 6.9% Sample 3 880 798 777 920 843 58.47 6.9%

Synonyms

Hepatitis A virus cellular receptor 1; HAVcr-1; Kidney injury molecule 1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIMD-1; T-cell immunoglobulin mucin receptor 1; TIM; TIM-1; T-cell membrane protein 1; HAVCR1; KIM1, TIM1, TIMD1

Gene Name

HAVCR1

Gene ID

26762

UniProt

Q96D42

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S21-T288

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses. .

Applications

ELISA

Field of Research

Adaptive Immunity, Host-Virus Interaction, Immune System Diseases, Immunology, Interspecies Interaction, Microbiology, Non-CD, T Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and urine.

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 215 880 93 208 802 Standard deviation 5.42 9.24 39.6 5.48 11.02 40.1 CV (%) 4.5% 4.3% 4.5% 5.9% 5.3% 5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 215 880 93 208 802 Standard deviation 5.42 9.24 39.6 5.48 11.02 40.1 CV (%) 4.5% 4.3% 4.5% 5.9% 5.3% 5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 92 215 880 93 208 802 Mean (pg/ml) 92 215 880 93 208 802 Standard deviation 5.42 9.24 39.6 5.48 11.02 40.1 Standard deviation 5.42 9.24 39.6 5.48 11.02 40.1 CV (%) 4.5% 4.3% 4.5% 5.9% 5.3% 5% CV (%) 4.5% 4.3% 4.5% 5.9% 5.3% 5%

Sensitivity

<2 pg/mL

Function

May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity) . In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

38720 MW

Specificity

Natural and recombinant human KIM1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Hepatitis A virus cellular receptor 1

Subcellular Location

Membrane ; Single-pass type I membrane protein .

Sequence Similarities

Belongs to the immunoglobulin superfamily. TIM family.

Protein Name

Hepatitis A virus cellular receptor 1

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.045 0.127 0.194 0.301 0.506 0.935 1.57 2.607 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.045 0.127 0.194 0.301 0.506 0.935 1.57 2.607 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.045 0.127 0.194 0.301 0.506 0.935 1.57 2.607 O.D. 0.045 0.127 0.194 0.301 0.506 0.935 1.57 2.607

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.