Rat KIM1 ELISA Kit PicoKine®

KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rat is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the rat Tim1 gene and database analysis, was mapped the HAVCR1 gene to 5q33.2.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 87 94 102 93 94 5.33 5.6% Sample 2 272 238 277 253 260 15.54 5.9% Sample 3 938 849 928 895 902 34.74 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 87 94 102 93 94 5.33 5.6% Sample 1 87 94 102 93 94 5.33 5.6% Sample 2 272 238 277 253 260 15.54 5.9% Sample 2 272 238 277 253 260 15.54 5.9% Sample 3 938 849 928 895 902 34.74 3.8% Sample 3 938 849 928 895 902 34.74 3.8%

Hepatitis A virus cellular receptor 1 homolog; HAVcr-1; Kidney injury molecule 1; KIM-1; T cell immunoglobulin and mucin domain-containing protein 1; TIMD-1; Havcr1; Kim1

Expressed at a low level in normal kidney but are increased dramatically in postischemic kidney. Expressed in proliferating bromodeoxyuridine-positive and dedifferentiated vimentin-positive epithelial cells in regenerating proximal tubules. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 87 272 938 86 293 1026 Standard deviation 3.82 20.67 59.09 3.95 23.14 84.13 CV (%) 4.4% 7.6% 7.9% 4.6% 7.9% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 87 272 938 86 293 1026 Standard deviation 3.82 20.67 59.09 3.95 23.14 84.13 CV (%) 4.4% 7.6% 7.9% 4.6% 7.9% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 87 272 938 86 293 1026 Mean (pg/ml) 87 272 938 86 293 1026 Standard deviation 3.82 20.67 59.09 3.95 23.14 84.13 Standard deviation 3.82 20.67 59.09 3.95 23.14 84.13 CV (%) 4.4% 7.6% 7.9% 4.6% 7.9% 8.2% CV (%) 4.4% 7.6% 7.9% 4.6% 7.9% 8.2%

May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.067 0.256 0.35 0.499 0.745 1.175 1.847 2.793 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.067 0.256 0.35 0.499 0.745 1.175 1.847 2.793 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.067 0.256 0.35 0.499 0.745 1.175 1.847 2.793 O.D. 0.067 0.256 0.35 0.499 0.745 1.175 1.847 2.793

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.