Human HSP27/HSPB1/Heat Shock Protein 27 ELISA Kit PicoKine®

Human HSP27 / Heat Shock Protein 27 ELISA Kit PicoKine® (96 Tests) . Quantitate Human HSPB1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, alpha-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7kDa.

Reproducibility

Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 88 78 91 84 5.06 6% Sample 2 492 526 485 501 501 15.5 3% Sample 3 1757 1541 1607 1549 1613 86.67 5.3% Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 88 78 91 84 5.06 6% Sample 1 82 88 78 91 84 5.06 6% Sample 2 492 526 485 501 501 15.5 3% Sample 2 492 526 485 501 501 15.5 3% Sample 3 1757 1541 1607 1549 1613 86.67 5.3% Sample 3 1757 1541 1607 1549 1613 86.67 5.3%

Synonyms

Heat shock protein beta-1; HspB1;28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27, HSP28

Gene Name

HSPB1

Gene ID

3315

UniProt

P04792

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T2-K205

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. .

Applications

ELISA

Field of Research

Contractility, Heat Shock Proteins, Protein Trafficking, Signal Transduction, Tumor Biomarkers

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL-4000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 492 1757 81 468 1729 Standard deviation 3.44 26.07 110.69 4.53 33.69 121.03 CV (%) 4.2% 5.3% 7.2%% 5.6% 7.2% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 492 1757 81 468 1729 Standard deviation 3.44 26.07 110.69 4.53 33.69 121.03 CV (%) 4.2% 5.3% 7.2%% 5.6% 7.2% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 82 492 1757 81 468 1729 Mean (pg/ml) 82 492 1757 81 468 1729 Standard deviation 3.44 26.07 110.69 4.53 33.69 121.03 Standard deviation 3.44 26.07 110.69 4.53 33.69 121.03 CV (%) 4.2% 5.3% 7.2%% 5.6% 7.2% 7% CV (%) 4.2% 5.3% 7.2%% 5.6% 7.2% 7%

Sensitivity

<5pg/mL

Function

Involved in stress resistance and actin organization.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

22783 MW

Specificity

Natural and recombinant human HSP27

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Heat shock protein beta-1

Subcellular Location

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.

Sequence Similarities

Belongs to the small heat shock protein (HSP20) family.

Protein Name

Heat shock protein beta-1

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.03 0.079 0.129 0.211 0.379 0.701 1.258 1.968 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.03 0.079 0.129 0.211 0.379 0.701 1.258 1.968 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.03 0.079 0.129 0.211 0.379 0.701 1.258 1.968 O.D. 0.03 0.079 0.129 0.211 0.379 0.701 1.258 1.968

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.