Mouse KIM1 / TIM1 ELISA Kit PicoKine®

KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene.[1][2][3] Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 87 90 90 96 90 3.26 3.6% Sample 2 279 266 249 284 269 13.53 5% Sample 3 842 734 811 767 788 41.23 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 87 90 90 96 90 3.26 3.6% Sample 1 87 90 90 96 90 3.26 3.6% Sample 2 279 266 249 284 269 13.53 5% Sample 2 279 266 249 284 269 13.53 5% Sample 3 842 734 811 767 788 41.23 5.2% Sample 3 842 734 811 767 788 41.23 5.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 87 279 842 85 261 792 Standard deviation 6.87 14.78 36.2 7.31 14.87 38.8 CV (%) 7.9% 5.3% 4.3% 8.6% 5.7% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 87 279 842 85 261 792 Standard deviation 6.87 14.78 36.2 7.31 14.87 38.8 CV (%) 7.9% 5.3% 4.3% 8.6% 5.7% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 87 279 842 85 261 792 Mean (pg/ml) 87 279 842 85 261 792 Standard deviation 6.87 14.78 36.2 7.31 14.87 38.8 Standard deviation 6.87 14.78 36.2 7.31 14.87 38.8 CV (%) 7.9% 5.3% 4.3% 8.6% 5.7% 4.9% CV (%) 7.9% 5.3% 4.3% 8.6% 5.7% 4.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.079 0.129 0.211 0.379 0.701 1.258 2.158 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.079 0.129 0.211 0.379 0.701 1.258 2.158 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.079 0.129 0.211 0.379 0.701 1.258 2.158 O.D. 0.03 0.079 0.129 0.211 0.379 0.701 1.258 2.158

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.