Human FLRG/FSTL3 ELISA Kit PicoKine®

Human FLRG/FSTL3 ELISA Kit PicoKine® (96 Tests) . Quantitate Human FSTL3 in cell culture supernatants, serum, plasma (heparin, EDTA), urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

FSTL3 (Follistatin-Like 3) also known as FLRG or FOLLISTATIN-RELATED GENE, is a member of the follistatin-module protein family, which is composed of extracellular matrix-associated glycoproteins thought to act in a paracrine manner to bind morphogens or growth/differentiation factors and regulate their activity during development. The FSTL3 gene extends over 7 kbp and contains 5 exons. The FSTL3 gene was localized to chromosome 19p13. This gene involved in hematopoiesis and in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. It may have a role in leukemogenesis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 141 134 152 132 139 7.82 5.6% Sample 2 737 760 710 697 726 24.36 3.3% Sample 3 1859 1978 1927 2009 1943 56.77 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 141 134 152 132 139 7.82 5.6% Sample 1 141 134 152 132 139 7.82 5.6% Sample 2 737 760 710 697 726 24.36 3.3% Sample 2 737 760 710 697 726 24.36 3.3% Sample 3 1859 1978 1927 2009 1943 56.77 2.9% Sample 3 1859 1978 1927 2009 1943 56.77 2.9%

Synonyms

Follistatin-related protein 3; Follistatin-like protein 3; Follistatin-related gene protein; FSTL3; FLRG; UNQ674/PRO1308

Gene Name

FSTL3

Gene ID

10272

UniProt

O95633

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M27-V263

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in a wide range of tissues. .

Applications

ELISA

Field of Research

Growth Factors/Hormones, Hematopoietic Progenitors, Lymphoid, Mesenchymal Stem Cells, Signal Transduction, Stem Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), urine and human milk.

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 141 737 1859 153 741 1910 Standard deviation 5.78 34.63 126.41 6.73 40.75 150.89 CV (%) 4.1% 4.7% 6.8% 4.4% 5.5% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 141 737 1859 153 741 1910 Standard deviation 5.78 34.63 126.41 6.73 40.75 150.89 CV (%) 4.1% 4.7% 6.8% 4.4% 5.5% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 141 737 1859 153 741 1910 Mean (pg/ml) 141 737 1859 153 741 1910 Standard deviation 5.78 34.63 126.41 6.73 40.75 150.89 Standard deviation 5.78 34.63 126.41 6.73 40.75 150.89 CV (%) 4.1% 4.7% 6.8% 4.4% 5.5% 7.9% CV (%) 4.1% 4.7% 6.8% 4.4% 5.5% 7.9%

Sensitivity

<10 pg/mL

Function

Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

27663 MW

Specificity

Natural and recombinant human FLRG

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Follistatin-related protein 3

Subcellular Location

Isoform 1: Secreted.

Sequence Similarities

Contains 2 follistatin-like domains.

Protein Name

Follistatin-related protein 3

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.04 0.099 0.176 0.352 0.592 0.951 1.384 1.833 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.04 0.099 0.176 0.352 0.592 0.951 1.384 1.833 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.04 0.099 0.176 0.352 0.592 0.951 1.384 1.833 O.D. 0.04 0.099 0.176 0.352 0.592 0.951 1.384 1.833

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.