Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine®

Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human DKK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 125 119 115 116 6.17 5.3% Sample 2 660 564 616 629 617 34.65 5.6% Sample 3 1140 1087 1155 1110 1123 26.35 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 125 119 115 116 6.17 5.3% Sample 1 108 125 119 115 116 6.17 5.3% Sample 2 660 564 616 629 617 34.65 5.6% Sample 2 660 564 616 629 617 34.65 5.6% Sample 3 1140 1087 1155 1110 1123 26.35 2.3% Sample 3 1140 1087 1155 1110 1123 26.35 2.3%

Synonyms

Dickkopf-like protein 1 ; DKK1

Gene Name

DKK1

Gene ID

22943

UniProt

I1W660

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T32-H266

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Developmental Biology, Embryogenesis, Immunology, Secreted, Signaling Pathways, Stem Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 660 1140 103 594 1134 Standard deviation 5.72 47.52 66.12 6.59 43.36 82.78 CV (%) 5.3% 7.2% 5.8% 6.4% 7.3% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 660 1140 103 594 1134 Standard deviation 5.72 47.52 66.12 6.59 43.36 82.78 CV (%) 5.3% 7.2% 5.8% 6.4% 7.3% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 108 660 1140 103 594 1134 Mean (pg/ml) 108 660 1140 103 594 1134 Standard deviation 5.72 47.52 66.12 6.59 43.36 82.78 Standard deviation 5.72 47.52 66.12 6.59 43.36 82.78 CV (%) 5.3% 7.2% 5.8% 6.4% 7.3% 7.3% CV (%) 5.3% 7.2% 5.8% 6.4% 7.3% 7.3%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

28672 MW

Specificity

Oligomeric forms of natural and recombinant human DKK1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Dickkopf WNT signaling pathway inhibitor 1

Protein Name

Dickkopf-related protein 1

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.07 0.113 0.218 0.358 0.628 1.048 1.96 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.07 0.113 0.218 0.358 0.628 1.048 1.96 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.07 0.113 0.218 0.358 0.628 1.048 1.96 O.D. 0.014 0.07 0.113 0.218 0.358 0.628 1.048 1.96

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.