Mouse IL-23 ELISA Kit PicoKine®

Interleukin-23 subunit alpha (IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19 (the IL-23 alpha subunit) . IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. he International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12 (stSG47812) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 84 99 94 92 5.4 5.8% Sample 2 278 287 274 301 285 10.36 3.6% Sample 3 780 941 844 875 860 57.96 6.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 84 99 94 92 5.4 5.8% Sample 1 93 84 99 94 92 5.4 5.8% Sample 2 278 287 274 301 285 10.36 3.6% Sample 2 278 287 274 301 285 10.36 3.6% Sample 3 780 941 844 875 860 57.96 6.7% Sample 3 780 941 844 875 860 57.96 6.7%

Secreted by activated dendritic cells (at protein level) . Detected in various tissues with higher expression in polarized Th1 cells and activated macrophages. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 278 780 85 279 762 Standard deviation 4.09 13.9 34.32 4.4%7 19.25 38.86 CV (%) 4.4% 5% 4.4% 6.2% 6.9% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 278 780 85 279 762 Standard deviation 4.09 13.9 34.32 4.4%7 19.25 38.86 CV (%) 4.4% 5% 4.4% 6.2% 6.9% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 93 278 780 85 279 762 Mean (pg/ml) 93 278 780 85 279 762 Standard deviation 4.09 13.9 34.32 4.4%7 19.25 38.86 Standard deviation 4.09 13.9 34.32 4.4%7 19.25 38.86 CV (%) 4.4% 5% 4.4% 6.2% 6.9% 5.1% CV (%) 4.4% 5% 4.4% 6.2% 6.9% 5.1%

Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak- Stat signaling cascade, stimulates memory rather than naive T- cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.043 0.115 0.175 0.268 0.446 0.823 1.418 2.273 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.043 0.115 0.175 0.268 0.446 0.823 1.418 2.273 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.043 0.115 0.175 0.268 0.446 0.823 1.418 2.273 O.D. 0.043 0.115 0.175 0.268 0.446 0.823 1.418 2.273

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.