Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine®

PTX3 (Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 262 259 249 272 260 8.2 3.1% Sample 2 1334 1350 1444 1511 1409 71.99 5.1% Sample 3 4146 4596 4000 4523 4316 250 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 262 259 249 272 260 8.2 3.1% Sample 1 262 259 249 272 260 8.2 3.1% Sample 2 1334 1350 1444 1511 1409 71.99 5.1% Sample 2 1334 1350 1444 1511 1409 71.99 5.1% Sample 3 4146 4596 4000 4523 4316 250 5.7% Sample 3 4146 4596 4000 4523 4316 250 5.7%

Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Neurodegenerative Disease, Neurogenesis, Neurology Process, Neuroscience, Transcription

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 262 1334 4146 264 1451 3917 Standard deviation 15.19 98.71 211.44 15.84 113.17 274.19 CV (%) 5.8% 7.4% 7% 6% 7.8% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 262 1334 4146 264 1451 3917 Standard deviation 15.19 98.71 211.44 15.84 113.17 274.19 CV (%) 5.8% 7.4% 7% 6% 7.8% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 262 1334 4146 264 1451 3917 Mean (pg/ml) 262 1334 4146 264 1451 3917 Standard deviation 15.19 98.71 211.44 15.84 113.17 274.19 Standard deviation 15.19 98.71 211.44 15.84 113.17 274.19 CV (%) 5.8% 7.4% 7% 6% 7.8% 7% CV (%) 5.8% 7.4% 7% 6% 7.8% 7%

Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self- components and female fertility. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.054 0.106 0.2 0.403 0.769 1.408 2.293 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.054 0.106 0.2 0.403 0.769 1.408 2.293 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.054 0.106 0.2 0.403 0.769 1.408 2.293 O.D. 0.004 0.054 0.106 0.2 0.403 0.769 1.408 2.293

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.