Human PTX3/Pentraxin 3 ELISA Kit PicoKine®

PTX3 (Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 662 620 693 604 644 34.99 5.4% Sample 2 1701 1556 1536 1612 1601 63.97 3.9% Sample 3 9194 8999 8940 8763 8974 153.86 1.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 662 620 693 604 644 34.99 5.4% Sample 1 662 620 693 604 644 34.99 5.4% Sample 2 1701 1556 1536 1612 1601 63.97 3.9% Sample 2 1701 1556 1536 1612 1601 63.97 3.9% Sample 3 9194 8999 8940 8763 8974 153.86 1.7% Sample 3 9194 8999 8940 8763 8974 153.86 1.7%

Pentraxin-related protein PTX3; Pentaxin-related protein PTX3; Tumor necrosis factor alpha-induced protein 5; TNF alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; TSG-14; PTX3; TNFAIP5, TSG14

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 662 1701 9194 718 1816 9601 Standard deviation 33.76 85.05 579.22 38.77 92.61 662.46 CV (%) 6.9% 5% 6.9% 5.4% 6.9% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 662 1701 9194 718 1816 9601 Standard deviation 33.76 85.05 579.22 38.77 92.61 662.46 CV (%) 6.9% 5% 6.9% 5.4% 6.9% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 662 1701 9194 718 1816 9601 Mean (pg/ml) 662 1701 9194 718 1816 9601 Standard deviation 33.76 85.05 579.22 38.77 92.61 662.46 Standard deviation 33.76 85.05 579.22 38.77 92.61 662.46 CV (%) 6.9% 5% 6.9% 5.4% 6.9% 6.9% CV (%) 6.9% 5% 6.9% 5.4% 6.9% 6.9%

Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self- components and female fertility. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.02 0.085 0.149 0.28 0.44 0.932 1.629 2.053 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.02 0.085 0.149 0.28 0.44 0.932 1.629 2.053 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.02 0.085 0.149 0.28 0.44 0.932 1.629 2.053 O.D. 0.02 0.085 0.149 0.28 0.44 0.932 1.629 2.053

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.