Human PAI-1 ELISA Kit PicoKine®

Human PAI-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human SERPINE1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots) . It is a serine protease inhibitor (serpin) protein (SERPINE1) . PAI-1 is mainly produced by the endothelium (cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on the seventh chromosome (7q21.3-q22) .1 Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis (a tendency to hemorrhage) . PAI-1 is present in increased levels in various disease states (such as a number of forms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1.2 The standard product used in this kit is recombinant human PAI-1 with the molecular mass of 43KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 218 218 234 218 10.96 5% Sample 2 1208 1308 1324 1403 1310 69.37 5.2% Sample 3 5496 5184 6077 5696 5613 324 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 218 218 234 218 10.96 5% Sample 1 203 218 218 234 218 10.96 5% Sample 2 1208 1308 1324 1403 1310 69.37 5.2% Sample 2 1208 1308 1324 1403 1310 69.37 5.2% Sample 3 5496 5184 6077 5696 5613 324 5.7% Sample 3 5496 5184 6077 5696 5613 324 5.7%

Synonyms

Plasminogen activator inhibitor 1; PAI; PAI-1; Endothelial plasminogen activator inhibitor; Serpin E1; SERPINE1; PAI1, PLANH1

Gene Name

SERPINE1

Gene ID

5054

UniProt

P05121

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S22-P402

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

Applications

ELISA

Field of Research

Angiogenesis, Cardiovascular

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 1208 5496 198 1244 5980 Standard deviation 12.38 71.27 423.19 15.04 95.78 520.26 CV (%) 6.1% 7.7%% 7.7% 7.6% 7.7% 8.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 1208 5496 198 1244 5980 Standard deviation 12.38 71.27 423.19 15.04 95.78 520.26 CV (%) 6.1% 7.7%% 7.7% 7.6% 7.7% 8.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 203 1208 5496 198 1244 5980 Mean (pg/ml) 203 1208 5496 198 1244 5980 Standard deviation 12.38 71.27 423.19 15.04 95.78 520.26 Standard deviation 12.38 71.27 423.19 15.04 95.78 520.26 CV (%) 6.1% 7.7%% 7.7% 7.6% 7.7% 8.7% CV (%) 6.1% 7.7%% 7.7% 7.6% 7.7% 8.7%

Sensitivity

<10 pg/mL

Function

Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

45060 MW

Specificity

Natural and recombinant human PAI-1

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Plasminogen activator inhibitor 1

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the serpin family.

Protein Name

Plasminogen activator inhibitor 1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.101 0.138 0.221 0.406 0.712 1.466 2.268 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.101 0.138 0.221 0.406 0.712 1.466 2.268 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.101 0.138 0.221 0.406 0.712 1.466 2.268 O.D. 0.055 0.101 0.138 0.221 0.406 0.712 1.466 2.268

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.