Human TNFSF4/OX40L ELISA Kit PicoKine®

Human TNFSF4/OX40L ELISA Kit PicoKine® (96 Tests) . Quantitate Human TNFSF4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, the mouse Tnfsf4 gene is mapped to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene. OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and that polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 209 197 197 202 201 4.91 2.4% Sample 2 1225 1132 1200 1122 1169 43.79 3.7% Sample 3 4641 5209 4997 5343 5047 265.13 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 209 197 197 202 201 4.91 2.4% Sample 1 209 197 197 202 201 4.91 2.4% Sample 2 1225 1132 1200 1122 1169 43.79 3.7% Sample 2 1225 1132 1200 1122 1169 43.79 3.7% Sample 3 4641 5209 4997 5343 5047 265.13 5.2% Sample 3 4641 5209 4997 5343 5047 265.13 5.2%

Synonyms

Tumor necrosis factor ligand superfamily member 4; Glycoprotein Gp34; OX40 ligand; OX40L; TAX transcriptionally-activated glycoprotein 1; CD252; TNFSF4; TXGP1

Gene Name

TNFSF4

Gene ID

7292

UniProt

P23510

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q51-L183

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Adaptive Immunity, Autoimmune, B Cells, Cell Type Markers, Cytokines, Immune System Diseases, Immunology, Innate Immunity, T Cells, TNF Superfamily

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 209 1225 4641 228 1277 4885 Standard deviation 8.56 51.45 305.70 12.54 72.78 365.77 CV (%) 4.1% 4.2% 6.6% 5.5% 5.7% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 209 1225 4641 228 1277 4885 Standard deviation 8.56 51.45 305.70 12.54 72.78 365.77 CV (%) 4.1% 4.2% 6.6% 5.5% 5.7% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 209 1225 4641 228 1277 4885 Mean (pg/ml) 209 1225 4641 228 1277 4885 Standard deviation 8.56 51.45 305.70 12.54 72.78 365.77 Standard deviation 8.56 51.45 305.70 12.54 72.78 365.77 CV (%) 4.1% 4.2% 6.6% 5.5% 5.7% 7.5% CV (%) 4.1% 4.2% 6.6% 5.5% 5.7% 7.5%

Sensitivity

<10 pg/mL

Function

Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

21050 MW

Specificity

Natural and recombinant human TNFSF4

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tumor necrosis factor ligand superfamily member 4

Subcellular Location

Membrane; Single-pass type II membrane protein.

Sequence Similarities

Belongs to the tumor necrosis factor family.

Protein Name

Tumor necrosis factor ligand superfamily member 4

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.101 0.19 0.316 0.557 1.018 1.751 2.543 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.101 0.19 0.316 0.557 1.018 1.751 2.543 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.101 0.19 0.316 0.557 1.018 1.751 2.543 O.D. 0.025 0.101 0.19 0.316 0.557 1.018 1.751 2.543

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.