Human Nidogen-1 / Entactin / NID-1 / NID1 ELISA Kit PicoKine®

Nidogen-1 (NID-1), also known as Entactin, is a protein that in humans is encoded by the NID1 gene. It is a member of the nidogen family of basement membrane glycoproteins. This gene is mapped to 1q42.3. Nidogen-1 is a component of the basement membrane alongside other components such as collagen type IV, proteoglycans (heparan sulfate and glycosaminoglycans), laminin and fibronectin. The protein interacts with several other components of basement membranes. Structurally it (along with perlecan) connects the networks formed by collagens and laminins to each other. It may also play a role in cell interactions with the extracellular matrix. Nidogen-1 also can serve as a bridge between the 2 most abundant molecules in the basement membrane: type IV collagen and laminin.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 132 145 147 147 142 6.25 4.4% Sample 2 391 380 383 427 395 18.76 4.7% Sample 3 1350 1536 1504 1575 1491 85.33 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 132 145 147 147 142 6.25 4.4% Sample 1 132 145 147 147 142 6.25 4.4% Sample 2 391 380 383 427 395 18.76 4.7% Sample 2 391 380 383 427 395 18.76 4.7% Sample 3 1350 1536 1504 1575 1491 85.33 5.7% Sample 3 1350 1536 1504 1575 1491 85.33 5.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 132 391 1350 121 413 1428 Standard deviation 5.41 17.98 76.95 6.65 26.43 108.52 CV (%) 4.1% 4.6% 5.7% 5.5% 6.4% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 132 391 1350 121 413 1428 Standard deviation 5.41 17.98 76.95 6.65 26.43 108.52 CV (%) 4.1% 4.6% 5.7% 5.5% 6.4% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 132 391 1350 121 413 1428 Mean (pg/ml) 132 391 1350 121 413 1428 Standard deviation 5.41 17.98 76.95 6.65 26.43 108.52 Standard deviation 5.41 17.98 76.95 6.65 26.43 108.52 CV (%) 4.1% 4.6% 5.7% 5.5% 6.4% 7.6% CV (%) 4.1% 4.6% 5.7% 5.5% 6.4% 7.6%

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell- extracellular matrix interactions.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.024 0.058 0.096 0.156 0.262 0.476 0.91 1.885 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.024 0.058 0.096 0.156 0.262 0.476 0.91 1.885 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.024 0.058 0.096 0.156 0.262 0.476 0.91 1.885 O.D. 0.024 0.058 0.096 0.156 0.262 0.476 0.91 1.885

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.