Human CD10/Neprilysin ELISA Kit PicoKine®

CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), Neprilysin, or common acute lymphoblastic leukemia antigen (CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 201 170 181 181 183 11.18 6.1% Sample 2 956 1058 1011 1064 1022 43.4 4.2% Sample 3 4316 4490 4532 4435 4443 81.12 1.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 201 170 181 181 183 11.18 6.1% Sample 1 201 170 181 181 183 11.18 6.1% Sample 2 956 1058 1011 1064 1022 43.4 4.2% Sample 2 956 1058 1011 1064 1022 43.4 4.2% Sample 3 4316 4490 4532 4435 4443 81.12 1.8% Sample 3 4316 4490 4532 4435 4443 81.12 1.8%

Neprilysin;3.4.24.11 ; Atriopeptidase; Common acute lymphocytic leukemia antigen; CALLA; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase; SFE; CD10; MME; EPN

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Tumor Biomarkers

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 201 2166 4316 216 881 4302 Standard deviation 14.87 51.62 233.06 17.92 60.78 305.44 CV (%) 7.4% 5.4% 5.4% 8.3% 6.9% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 201 2166 4316 216 881 4302 Standard deviation 14.87 51.62 233.06 17.92 60.78 305.44 CV (%) 7.4% 5.4% 5.4% 8.3% 6.9% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 201 2166 4316 216 881 4302 Mean (pg/ml) 201 2166 4316 216 881 4302 Standard deviation 14.87 51.62 233.06 17.92 60.78 305.44 Standard deviation 14.87 51.62 233.06 17.92 60.78 305.44 CV (%) 7.4% 5.4% 5.4% 8.3% 6.9% 7.1% CV (%) 7.4% 5.4% 5.4% 8.3% 6.9% 7.1%

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535) . Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991) . Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675) . Involved in the degradation of atrial natriuretic factor (ANF) (PubMed:2531377, PubMed:2972276) . Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.013 0.062 0.102 0.189 0.356 0.743 1.327 2.021 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.013 0.062 0.102 0.189 0.356 0.743 1.327 2.021 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.013 0.062 0.102 0.189 0.356 0.743 1.327 2.021 O.D. 0.013 0.062 0.102 0.189 0.356 0.743 1.327 2.021

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.